微乳液液相色谱法同时测定渗透泵片中氢氯噻嗪和氯沙坦钾的含量
Simultaneous Determination of Hydrochlorothiazide and Losartan Potassium in Osmotic Pump Tablets by Microemulsion Liquid Chromatography.
作者信息
Li Liangxing, Lai Caiyun, Xuan Xueyi, Gao Chongkai, Li Ning
机构信息
Department of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Department of Pharmaceutical Analysis, School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
出版信息
J Chromatogr Sci. 2016 Sep;54(8):1415-20. doi: 10.1093/chromsci/bmw101. Epub 2016 Jun 21.
A rapid and efficient oil-in-water microemulsion liquid chromatographic (MELC) method has been optimized and validated for the determination of hydrochlorothiazide (HCT) and losartan potassium (LOP) in osmotic pump tablets. Samples were injected into a C18 (150 mm × 4.6 mm ID, 5 µm particle size) analytical column, which was maintained at 30°C. The most effective MELC system had a mobile phase consisting of 95% (v/v) of 3.0% (w/w) SDS, 6.0% (w/w) n-butanol, 0.8% (w/w) n-octane, 90.2% (w/w) water and 5% (v/v) acetonitrile (pH 5). The flow rate was 1.0 mL min(-1) and UV detection was performed at 265 nm. Linearity ranged from 2.5 to 12.5 µg mL(-1) for HCT and 10.0-60.0 µg mL(-1) for LOP (r > 0.999 for both drugs). The proposed method was rapid, precise (RSDs < 1.4%) and accurate (98.9% recovery for HCT and 101% recovery for LOP). It is applicable to simultaneous determination of HCT and LOP in osmotic pump tablets.
已优化并验证了一种快速高效的水包油微乳液液相色谱（MELC）方法，用于测定渗透泵片中的氢氯噻嗪（HCT）和氯沙坦钾（LOP）。将样品注入C18（150 mm×4.6 mm内径，5 µm粒径）分析柱，该柱保持在30°C。最有效的MELC系统的流动相由95%（v/v）的3.0%（w/w）十二烷基硫酸钠、6.0%（w/w）正丁醇、0.8%（w/w）正辛烷、90.2%（w/w）水和5%（v/v）乙腈（pH 5）组成。流速为1.0 mL min(-1)，在265 nm处进行紫外检测。HCT的线性范围为2.5至12.5 µg mL(-1)，LOP的线性范围为10.0 - 60.0 µg mL(-1)（两种药物的r均>0.999）。所提出的方法快速、精确（相对标准偏差<1.4%）且准确（HCT的回收率为98.9%，LOP的回收率为101%）。它适用于同时测定渗透泵片中的HCT和LOP。
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