催乳素对大鼠主动脉平滑肌中蛋白激酶C活性的刺激作用。

Prolactin stimulation of protein kinase C activity in rat aortic smooth muscle.

作者信息

Sauro M D, Buckley A R, Russell D H, Fitzpatrick D F

机构信息

Department of Pharmacology and Therapeutics University of South Florida, College of Medicine, Tampa 33612.

出版信息

Life Sci. 1989;44(23):1787-92. doi: 10.1016/0024-3205(89)90566-3.

DOI:10.1016/0024-3205(89)90566-3

PMID:2733552

Abstract

Prolactin (PRL) activated protein kinase C (PKC) in a dose dependent manner in rat aortic smooth muscle. Aortic strips incubated with sub-nanomolar concentrations of ovine PRL for 25 min. at 37 degrees C showed a significant stimulation of PKC activity in both cytosolic and particulate fractions. This activation could be blocked using either anti-PRL antibodies or 1-(5- isoquinolinesulfonyl)-2-methylpiperazine (H-7), a PKC inhibitor. The results further support the role of PKC in the signal transduction pathway for PRL action and suggest that this activation may be involved in vascular smooth muscle function.

摘要

催乳素（PRL）在大鼠主动脉平滑肌中以剂量依赖方式激活蛋白激酶C（PKC）。将主动脉条与亚纳摩尔浓度的羊催乳素在37摄氏度下孵育25分钟。结果显示，细胞溶质和微粒部分中的PKC活性均受到显著刺激。使用抗PRL抗体或PKC抑制剂1-（5-异喹啉磺酰基）-2-甲基哌嗪（H-7）均可阻断这种激活。这些结果进一步支持了PKC在PRL作用的信号转导途径中的作用，并表明这种激活可能参与血管平滑肌功能。