Russo A, Pacchierotti F, Bassani B, Levis A G
Department of Biology, University of Padova, Italy.
Mutat Res. 1989 Jun;226(2):111-4. doi: 10.1016/0165-7992(89)90052-3.
Nitrilotriacetic acid (NTA) was tested for the induction of aneuploidy in mouse bone marrow cells. Doses of 138 or 275 mg/kg of body weight were intraperitoneally injected 24 h after implantation of a bromodeoxyuridine tablet. Cell-replication kinetics was assessed by comparing the relative percentages of first, second and third metaphases in control and treated samples. The hyperploidy incidence was estimated in second metaphases only, together with the SCE/cell level. Mice injected with 1.8 mg/kg vinblastine (VBL) were used as positive controls. A slight delay of cell cycle was induced by NTA, as shown by regression analysis applied to average generation time values. No increase over the control level was observed for hyperploidy or SCE induction in NTA-treated mice. VBL induced both cell-cycle alteration and a highly significant (P less than 0.001) increase of the hyperploid cell frequency. On the basis of these and previous (Costa et al., 1988) observations it seems that the non-disjunctional activity of NTA in the mouse is confined to meiotic processes.
对次氮基三乙酸（NTA）诱导小鼠骨髓细胞非整倍体的情况进行了测试。在植入溴脱氧尿苷片24小时后，腹腔注射剂量为138或275毫克/千克体重的NTA。通过比较对照样品和处理样品中第一、第二和第三中期的相对百分比来评估细胞复制动力学。仅在第二中期估计超倍体发生率以及每个细胞的姐妹染色单体交换（SCE）水平。注射1.8毫克/千克长春碱（VBL）的小鼠用作阳性对照。应用于平均世代时间值的回归分析表明，NTA诱导了细胞周期的轻微延迟。在经NTA处理的小鼠中，未观察到超倍体或SCE诱导率超过对照水平。VBL既诱导了细胞周期改变，又使超倍体细胞频率显著增加（P小于0.001）。基于这些以及之前（科斯塔等人，1988年）的观察结果，似乎NTA在小鼠中的不分离活性仅限于减数分裂过程。
