Gustavino B, Bassani B, Pacchierotti F
Laboratory of Toxicology, ENEA, CRE Casaccia, Rome, Italy.
Mutat Res. 1991 May;248(1):45-50. doi: 10.1016/0027-5107(91)90086-4.
The effect of selection processes operating on chemically induced aneuploid and polyploid cells was studied in mouse bone marrow cells at their third generation after a single i.p. treatment with vinblastine (VBL). Bromodeoxyuridine (BrdUrd)-labeled metaphases were analyzed for chromosome number and the frequencies of aneuploid and polyploid cells recorded at 2 different times, both within the third cell cycle after VBL treatment. Cell-cycle progression was analyzed for both control and treated mice at the 2 fixation times. Our data suggest that polyploid cells and possibly also cells with numerous additional chromosomes could have a cell cycle longer than that of diploid cells and cells hyperploid for 1-2 additional chromosomes. Both hyperploid and polyploid cells seem to have a reduced probability of undergoing further mitoses, as shown by the reduction of their frequencies at the third cell cycle, when compared to the frequencies observed in the second cell cycle after the same VBL treatment.
在用长春碱（VBL）单次腹腔注射处理后的第三代小鼠骨髓细胞中，研究了选择过程对化学诱导的非整倍体和多倍体细胞的影响。分析了溴脱氧尿苷（BrdUrd）标记的中期细胞的染色体数目，并在VBL处理后的第三个细胞周期内的2个不同时间记录非整倍体和多倍体细胞的频率。在2个固定时间对对照小鼠和处理小鼠的细胞周期进程进行了分析。我们的数据表明，多倍体细胞以及可能还有具有大量额外染色体的细胞，其细胞周期可能比二倍体细胞和超二倍体1 - 2条额外染色体的细胞更长。与相同VBL处理后第二个细胞周期观察到的频率相比，超二倍体和多倍体细胞在第三个细胞周期时频率降低，这表明它们进行进一步有丝分裂的概率似乎降低。
