Scaramuzzi Karina, Tanaka Gabriela D, Neto Francisco Mariano, Garcia Paulo R A F, Gabrili Joel J M, Oliveira Denise C A, Tambourgi Denise V, Mussalem Juliana S, Paixão-Cavalcante Danielle, D'Azeredo Orlando Marcos T, Botosso Viviane F, Oliveira Cristiano L P, Fantini Márcia C A, Sant'Anna Osvaldo A
Immunochemistry Laboratory, Butantan Institute, São Paulo, Brazil.
Physics Institute, University of São Paulo, São Paulo, Brazil.
Nanomedicine. 2016 Nov;12(8):2241-2250. doi: 10.1016/j.nano.2016.06.003. Epub 2016 Jun 23.
Due to its physicochemical properties, nanostructured mesoporous SBA-15 silica shows great potential as a vaccine adjuvant. This study evaluated the capacity of SBA-15 to encapsulate/adsorb the recombinant purified HBsAg from the Hepatitis B virus and the immunoresponsiveness of mice orally immunized with HBsAg inside SBA-15. A simulation of small angle X-ray scattering experimental results, together with the nitrogen adsorption isotherms data, allowed to determine the appropriate mass ratio of HBsAg:SBA-15, indicating antigen encapsulation into SBA-15 macroporosity. This was also evaluated by bicinchoninic acid assay and gel electrophoresis. The recruitment of inflammatory cells, an increase in production of specific antibodies, and the non-influence of silica on T1 or T2 polarization were observed after oral immunization. Besides, SBA-15 enhanced the phagocytosis of ovalbumin by dendritic cells, an important key to prove how this adjuvant works. Thus, it seems clear that the nanostructured SBA-15 is an effective and safe adjuvant for oral immunizations.
由于其物理化学性质，纳米结构的介孔SBA-15二氧化硅作为疫苗佐剂显示出巨大潜力。本研究评估了SBA-15包封/吸附来自乙型肝炎病毒的重组纯化HBsAg的能力，以及用SBA-15内的HBsAg经口免疫小鼠的免疫反应性。小角X射线散射实验结果的模拟以及氮吸附等温线数据，有助于确定HBsAg:SBA-15的合适质量比，表明抗原被包封在SBA-15的大孔中。这也通过二辛可宁酸测定法和凝胶电泳进行了评估。经口免疫后观察到炎症细胞的募集、特异性抗体产生的增加以及二氧化硅对T1或T2极化无影响。此外，SBA-15增强了树突状细胞对卵清蛋白的吞噬作用，这是证明这种佐剂作用机制的一个重要关键。因此，很明显纳米结构的SBA-15是一种用于经口免疫的有效且安全的佐剂。
