Megnis Kaspars, Mandrika Ilona, Petrovska Ramona, Stukens Janis, Rovite Vita, Balcere Inga, Jansone Laima Sabine, Peculis Raitis, Pirags Valdis, Klovins Janis
Latvian Biomedical Research and Study Centre, Ratsupites Street 1 k-1, Riga LV-1067, Latvia.
Clinic of Neurosurgery, Pauls Stradins Clinical University Hospital, 13 Pilsonu Street, Riga LV-1002, Latvia.
Stem Cells Int. 2016;2016:7103720. doi: 10.1155/2016/7103720. Epub 2016 Jun 2.
Pituitary adenomas are one of the most common endocrine and intracranial neoplasms. Although they are theoretically monoclonal in origin, several studies have shown that they contain different multipotent cell types that are thought to play an important role in tumor initiation, maintenance, and recurrence after therapy. In the present study, we isolated and characterized cell populations from seven pituitary somatotroph, nonhormonal, and lactotroph adenomas. The obtained cells showed characteristics of multipotent mesenchymal stromal cells as observed by cell morphology, cell surface marker CD90, CD105, CD44, and vimentin expression, as well as differentiation to osteogenic and adipogenic lineages. They are capable of growth and passaging under standard laboratory cell culture conditions and do not manifest any hormonal cell characteristics. Multipotent mesenchymal stromal cells are present in pituitary adenomas regardless of their clinical manifestation and show no considerable expression of somatostatin 1-5 and dopamine 2 receptors. Most likely obtained cells are a part of tissue-supportive cells in pituitary adenoma microenvironment.
垂体腺瘤是最常见的内分泌和颅内肿瘤之一。尽管从理论上讲它们起源于单克隆，但多项研究表明，它们包含不同的多能细胞类型，这些细胞类型被认为在肿瘤的起始、维持以及治疗后的复发中起重要作用。在本研究中，我们从七个垂体生长激素细胞型、无激素型和催乳素细胞型腺瘤中分离并鉴定了细胞群体。通过细胞形态、细胞表面标志物CD90、CD105、CD44以及波形蛋白表达观察到，所获得的细胞表现出多能间充质基质细胞的特征，并且能够向成骨和成脂谱系分化。它们能够在标准实验室细胞培养条件下生长和传代，并且不表现出任何激素细胞特征。无论临床表现如何，垂体腺瘤中都存在多能间充质基质细胞，并且它们不大量表达生长抑素1-5和多巴胺2受体。最有可能的是，所获得的细胞是垂体腺瘤微环境中组织支持细胞的一部分。
