Steward A M
Aust N Z J Surg. 1977 Oct;47(5):642-7. doi: 10.1111/j.1445-2197.1977.tb06596.x.
Major advances in the understanding of clinical tumour biology occurred with the appreciation that tumour-associated substances circulated in the blood of cancer patients. In this study their origin and immunogenic function have been investigated. Whole tumour cells (WTC) and cancer cell membrane fractions (CMF) of 24 patients with lung, colon, and breast cancer were investigated for their antigenic effect upon the patients' own lymphocytes and upon healthy allogeneic ones. The antigenicity of whole lung and breast cancer cells to stimulate lymphocyte DNA synthesis, and the ineffectiveness of colon cells were confirmed. CMF had little stimulating effect upon autologous lymphocytes; however, they were able to augment lymphocyte response to PPD and PHA in high dilution and to suppress it in high concentration. The serum of cancer patients exerted similar biphasic activity upon PPD and PHA stimulated lymphocytes ("lymphosuppressive-stimulatory factors", or LSSF). Sephadex studies confirmed that LSSF activity in cancer serum correlated with circulating CMF. These substances modulate lymphocyte nucleic acid synthesis in vitro; it is likely that they similarly modulate the patient tumour-host cell relationship.
随着认识到肿瘤相关物质在癌症患者血液中循环，对临床肿瘤生物学的理解取得了重大进展。在本研究中，对它们的起源和免疫原性功能进行了调查。研究了24例肺癌、结肠癌和乳腺癌患者的全肿瘤细胞（WTC）和癌细胞膜组分（CMF）对患者自身淋巴细胞以及健康异体淋巴细胞的抗原作用。证实了全肺癌和乳腺癌细胞刺激淋巴细胞DNA合成的抗原性，以及结肠癌细胞的无效性。CMF对自体淋巴细胞的刺激作用很小；然而，它们能够在高稀释度下增强淋巴细胞对PPD和PHA的反应，并在高浓度下抑制该反应。癌症患者的血清对PPD和PHA刺激的淋巴细胞表现出类似的双相活性（“淋巴细胞抑制-刺激因子”，或LSSF）。葡聚糖凝胶研究证实，癌症血清中的LSSF活性与循环CMF相关。这些物质在体外调节淋巴细胞核酸合成；它们很可能同样调节患者肿瘤与宿主细胞的关系。
