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从外周血培养出的肿瘤球数量是乳腺癌患者发生转移的一个预测指标。

The number of tumorspheres cultured from peripheral blood is a predictor for presence of metastasis in patients with breast cancer.

作者信息

Pizon Monika, Schott Dorothea, Pachmann Ulrich, Pachmann Katharina

机构信息

Transfusion Center Bayreuth, 95448, Bayreuth, Germany.

出版信息

Oncotarget. 2016 Jul 26;7(30):48143-48154. doi: 10.18632/oncotarget.10174.

DOI:10.18632/oncotarget.10174
PMID:27340862
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5217007/
Abstract

BACKGROUND

Tumor metastases are the major cause of cancer morbidity and mortality. A subpopulation of tumor cells with stem-like properties is assumed to be responsible for tumor invasion, metastasis, heterogeneity and therapeutic resistance. This population is termed cancer stem cells (CSCs). We have developed a simple method for identification and characterization of circulating cancer stem cells among circulating epithelial tumor cells (CETCs).

METHODS

CETCs were cultured under conditions favoring growth of tumorspheres from 72 patients with breast cancer, including a subpopulation of 23 patients with metastatic disease. CETCs were determined using the maintrac® method. Gene expression profiles of single CETCs and tumorspheres of the same patients were analyzed using qRT-PCR.

RESULTS

Sphere formation was observed in 79 % of patients. We found that the number of tumorspheres depended on stage of disease. Furthermore, the most important factor for growing of tumorspheres is obtaining chemotherapy. Patients with chemotherapy treatment had lower numbers of tumorspheres compared to patients without chemotherapy. Patients with HER2 positive primary tumor had higher number of tumorspheres. Analysis of surface marker expression profile of tumorspheres showed that cells in the spheres had typical phenotype of cancer stem cells. There was no sphere formation in a control group with 50 healthy donors.

CONCLUSIONS

This study demonstrates that a small fraction of CETCs has proliferative activity. Identifying the CETC subset with cancer stem cell properties may provide more clinically useful prognostic information. Chemotherapy is the most important component in cancer therapy because it frequently reduces the number of tumorspheres.

摘要

背景

肿瘤转移是癌症发病和死亡的主要原因。具有干细胞样特性的肿瘤细胞亚群被认为是肿瘤侵袭、转移、异质性和治疗抵抗的原因。这个群体被称为癌症干细胞（CSCs）。我们已经开发出一种简单的方法来识别和表征循环上皮肿瘤细胞（CETCs）中的循环癌症干细胞。

方法

对72例乳腺癌患者的CETCs在有利于肿瘤球生长的条件下进行培养，其中包括23例转移性疾病患者亚群。使用maintrac®方法确定CETCs。使用qRT-PCR分析同一患者单个CETCs和肿瘤球的基因表达谱。

结果

79%的患者观察到球体形成。我们发现肿瘤球的数量取决于疾病阶段。此外，肿瘤球生长的最重要因素是接受化疗。与未接受化疗的患者相比，接受化疗的患者肿瘤球数量较少。HER2阳性原发性肿瘤患者的肿瘤球数量较多。肿瘤球表面标志物表达谱分析表明，球体中的细胞具有癌症干细胞的典型表型。50名健康供者的对照组未观察到球体形成。

结论

本研究表明一小部分CETCs具有增殖活性。识别具有癌症干细胞特性的CETC亚群可能提供更具临床实用性的预后信息。化疗是癌症治疗中最重要的组成部分，因为它经常减少肿瘤球的数量。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3b4/5217007/026e40f9bd06/oncotarget-07-48143-g012.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3b4/5217007/9c221210c17c/oncotarget-07-48143-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3b4/5217007/026e40f9bd06/oncotarget-07-48143-g012.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3b4/5217007/8796a3ae9fbc/oncotarget-07-48143-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3b4/5217007/9c221210c17c/oncotarget-07-48143-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3b4/5217007/01605bf8c1ac/oncotarget-07-48143-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3b4/5217007/e7009ab895da/oncotarget-07-48143-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b3b4/5217007/58449d42115a/oncotarget-07-48143-g011.jpg
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本文引用的文献

1
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2
Circulating cancer stem cells: the importance to select.循环肿瘤干细胞：选择的重要性。
Chin J Cancer Res. 2015 Oct;27(5):437-49. doi: 10.3978/j.issn.1000-9604.2015.04.08.
3
Spherical cancer models in tumor biology.肿瘤生物学中的球形癌症模型。
鸡胚绒毛尿囊膜（CAM）检测作为乳腺癌患者循环癌干细胞（cCSCs）来源的患者衍生异种移植模型。
Cancers (Basel). 2022 Mar 14;14(6):1476. doi: 10.3390/cancers14061476.
4
Circulating Tumor Cells in Hepatocellular Carcinoma: A Comprehensive Review and Critical Appraisal.循环肿瘤细胞在肝细胞癌中的研究进展：全面综述与批判性评价
Int J Mol Sci. 2021 Dec 3;22(23):13073. doi: 10.3390/ijms222313073.
5
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6
Colon adenocarcinoma-derived cells that express induced-pluripotent stem cell markers possess stem cell function.表达诱导多能干细胞标志物的结肠腺癌衍生细胞具有干细胞功能。
PLoS One. 2020 May 19;15(5):e0232934. doi: 10.1371/journal.pone.0232934. eCollection 2020.
7
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9
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