Chang A C, Williams K L, Williams J G, Ceccarelli A
School of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
Nucleic Acids Res. 1989 May 25;17(10):3655-61. doi: 10.1093/nar/17.10.3655.
A cDNA encoding mouse thymidylate synthase has been inserted 3' to the Dictyostelium discoideum actin 15 promoter in an E. coli-D.discoideum shuttle vector. When this construct was introduced into a D.discoideum thymidylate synthase mutant strain HPS400, stable transformants were obtained at high frequency. These transformants grew in standard axenic medium without requiring exogenous thymidine. This construct provides a second selectable marker for use in transformation of D.discoideum.
一个编码小鼠胸苷酸合成酶的cDNA已被插入到大肠杆菌-盘基网柄菌穿梭载体中盘基网柄菌肌动蛋白15启动子的3'端。当将此构建体导入盘基网柄菌胸苷酸合成酶突变株HPS400时，以高频率获得了稳定的转化体。这些转化体在标准的无细胞培养基中生长，无需外源胸苷。该构建体为盘基网柄菌的转化提供了第二个选择标记。
