A simplified kidney preservation system: the use of hyperosmolar perfusates for continuous perfusion preservation of canine kidneys.

Since the quality of renal preservation following simple ice storage is improved by the use of hyperosmolar washout solutions, hyperosmolar perfusates were evaluated for continuous perfusion preservation. Canine kidneys were preserved for 48 hours by continuous perfusion at 5 degrees C, using hyperosmolar cryoprecipitated plasma and 5% albumin perfusates of osmolalities 397 to 430 mOsm/kg H2O. Hyperosmolar plasma gave significantly better preservation than isosmolar plasma, but the results were only marginally superior to those already obtainable with isosmolar albumin solution. No further improvement in preservation by albumin perfusion was obtained with the hyperosmolar formulations. Because isosmolar albumin solution is easier to prepare than hyperosmolar cryoprecipitated plasma and gives comparable results, it remains our perfusate of choice for continuous perfusion preservation.