Toledo-Pereyra L H, Simmons R L, Najarian J S
Ann Surg. 1974 Nov;180(5):780-2. doi: 10.1097/00000658-197411000-00012.
Canine kidneys were autotransplanted into nephrectomized dogs after exposure to room temperature for 40-90 minutes and preservation for 24 hours by hypothermic bloodless pulsatile perfusiin. Three perfusates were used; cryoprecipitated pooled dogs' plasma, human albumin, and human plasmanate. Perfusion after periods of warm ischemia resulted in irreversible renal damage, unless allopurinol was added to the perfusate and fed to the dogs. Thus, metabolic manipulation is capable of protecting tissues in the face of severe ischemic insult, even when using plasma substitutes for perfusion.
将犬肾在室温下暴露40 - 90分钟后，通过低温无血搏动灌注保存24小时，然后自体移植到肾切除的犬体内。使用了三种灌注液：冷沉淀混合犬血浆、人白蛋白和人血浆代用品。除非在灌注液中加入别嘌呤醇并喂给犬，否则在经历一定时间的热缺血后进行灌注会导致不可逆的肾损伤。因此，即使在使用血浆代用品进行灌注时，代谢调控也能够在严重缺血损伤情况下保护组织。
