[骨髓增生异常综合征患者骨髓基质细胞IL-32 mRNA表达及其与细胞凋亡的相关性]

[IL-32 mRNA Expression of Bone Marrow Stromal Cells and Its Correlation with Cell Apoptosis in Patients with Myelodysplastic Syndrome].

作者信息

Zhang Yuan-Yu, Xu Li, Li Da-Qi, Shao Jian-Hua, Chen Ping, Zhao Hong-Yu, Dong Xue-Bin, Gu Lin-Ping, Wu Wei

机构信息

Weifang Medical College, Weifang 261000, Shandong Province, China.

Department of Hematology of Jinan Central Hospital Affiliated to Shandong University, Jinan 251000, Shandong Province, China.

出版信息

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2016 Jun;24(3):773-8. doi: 10.7534/j.issn.1009-2137.2016.03.026.

DOI:10.7534/j.issn.1009-2137.2016.03.026

PMID:27342508

Abstract

OBJECTIVE

To investigate the IL-32 mRNA expression of bone marrow stromal cells and its correlation with apoptosis of bone marrow mononuclear cells in patients with myelodysplastic syndrome (MDS).

METHODS

Bone marrow samples from 26 MDS patients and 10 iron deficiency anemia (IDA, as control) patients were collected, RT-PCR was used to detect the IL-32 mRNA expression of bone marrow stromal cells, and the apoptosis of bone marrow mononuclear cells was detected by flow cytometry with Annexin V-FITC/PI dowble staining. The born marrow lymphocytes and NK cells were detected by means of direct immunofluorescence labeling whole blood hemolysis and flow cytometry.

RESULTS

IL-32 mRNA expression of bone marrow stromal cells in the MDS patients was significantly higher than that of control group, the IL-32 mRNA expression of bone marrow stromal cells in patients with RA, RAS and RCMD was significantly higher than that in patients with RAEB. There was no obvious difference between RAEB and the control groups. The apoptosis of bone marrow mononuclear cells in MDS group was significantly higher than that in the control group, the apoptosis of bone marrow mononuclear cells in patients with RA, RAS and RCMD was significantly higher than that in RAEB. There was no significant difference between RAEB group and control group. The IL-32 mRNA expression in bone marrow stromal cells significantly correlated with the apoptosis of bone marrow mononuclear cells in MDS patients. The NK cell number in born marrow of MDS patients and the control group had no significant difference.

CONCLUSION

The expression of IL-32 mRNA in bone marrow stromal cells significantly relates with the apoptosis of MDS cells, and the secretion of IL-32 by bone marrow stromal cells may be one of the reasons for the apoptosis of MDS bone marrow cells. It is speculated that the abnormal MDS bone marrow microenvironment is involved in the apoptosis of bone marrow cells.

摘要

目的

探讨骨髓增生异常综合征（MDS）患者骨髓基质细胞白细胞介素-32（IL-32）mRNA表达及其与骨髓单个核细胞凋亡的相关性。

方法

采集26例MDS患者及10例缺铁性贫血（IDA，作为对照）患者的骨髓标本，采用逆转录聚合酶链反应（RT-PCR）检测骨髓基质细胞IL-32 mRNA表达，采用膜联蛋白V-异硫氰酸荧光素/碘化丙啶（Annexin V-FITC/PI）双染流式细胞术检测骨髓单个核细胞凋亡。采用直接免疫荧光标记全血溶血流式细胞术检测骨髓淋巴细胞及自然杀伤（NK）细胞。

结果

MDS患者骨髓基质细胞IL-32 mRNA表达显著高于对照组，难治性贫血（RA）、环形铁粒幼细胞性难治性贫血（RAS）及难治性血细胞减少伴多系发育异常（RCMD）患者骨髓基质细胞IL-32 mRNA表达显著高于难治性贫血伴原始细胞增多（RAEB）患者，RAEB患者与对照组比较差异无统计学意义。MDS组骨髓单个核细胞凋亡率显著高于对照组，RA、RAS及RCMD患者骨髓单个核细胞凋亡率显著高于RAEB患者，RAEB组与对照组比较差异无统计学意义。MDS患者骨髓基质细胞IL-32 mRNA表达与骨髓单个核细胞凋亡显著相关。MDS患者与对照组骨髓NK细胞数量比较差异无统计学意义。