Yang Shi-Mei, Lu Shi-Feng, Fei Xiao-Ming, Zhang Jian-Fu, Shen Wen-Yi, Li Jian-Yong, Lu Hua
Department of Hematology, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Dec;18(6):1560-3.
This study was aimed to investigate the expressions of multiple cytokines on bone marrow mesenchymal stem cells (MSC) in patients with aplastic anemia (AA) and myelodysplastic syndrome (MDS), and its significance. The semi-quantitative reverse transcriptase-PCR (RT-PCR) was used to detect the expressions of IL-1β, SCF, G-CSF at mRNA level in bone marrow MSC of patients with AA and MDS. The real time quantitative polymerase chain reaction (RQ-PCR) technique was used to detect the mRNA expression of TPO in bone marrow MSC of AA and MDS patients. The results indicated that the expression of SCF in AA group was much lower than that in the normal control group (p < 0.05), and the expression of TPO in AA group was higher than that in the normal control group (p < 0.05), while the expression of IL-1β of AA had no significant difference when compared with the normal control group (p > 0.05). Compared with normal control group, the expressions of SCF of MDS patients was lower (p < 0.05), but the expressions of IL-1β and TPO did not show significant difference (p > 0.05). The expressions of IL-1β, SCF and TPO were no significant difference between AA and MDS groups (p > 0.05). Neither the AA patients, MDS patients nor the normal control group had the expression of G-CSF. It is concluded that the expression of SCF and TPO in bone marrow MSC of AA patients are obviously abnormal, the expression of SCF is also abnormal in bone marrow MSC of MDS patients.
本研究旨在探讨再生障碍性贫血（AA）和骨髓增生异常综合征（MDS）患者骨髓间充质干细胞（MSC）中多种细胞因子的表达情况及其意义。采用半定量逆转录聚合酶链反应（RT-PCR）检测AA和MDS患者骨髓MSC中IL-1β、SCF、G-CSF在mRNA水平的表达。采用实时定量聚合酶链反应（RQ-PCR）技术检测AA和MDS患者骨髓MSC中TPO的mRNA表达。结果显示，AA组SCF的表达明显低于正常对照组（p<0.05），AA组TPO的表达高于正常对照组（p<0.05），而AA组IL-1β的表达与正常对照组相比无显著差异（p>0.05）。与正常对照组相比，MDS患者SCF的表达较低（p<0.05），但IL-1β和TPO的表达无显著差异（p>0.05）。AA组与MDS组IL-1β、SCF和TPO的表达无显著差异（p>0.05）。AA患者、MDS患者及正常对照组均无G-CSF表达。结论：AA患者骨髓MSC中SCF和TPO的表达明显异常，MDS患者骨髓MSC中SCF的表达也异常。
