Vargas E, Ruiz M A, Ferrero F J, Campuzano S, Ruiz-Valdepeñas Montiel V, Reviejo A J, Pingarrón J M
Departamento de Química Analítica, Facultad de CC. Químicas, Universidad Complutense de Madrid, Avda. Complutense s/n, 28040 Madrid, Spain; InBea Biosensores S.L. Parque Científico de Madrid, C/ Santiago Grisolía 2, 28760 Tres Cantos, Madrid.
InBea Biosensores S.L. Parque Científico de Madrid, C/ Santiago Grisolía 2, 28760 Tres Cantos, Madrid.
Talanta. 2016 Sep 1;158:6-13. doi: 10.1016/j.talanta.2016.05.050. Epub 2016 May 17.
A new automatic bioanalyzer for L-malic acid using an integrated amperometric biosensor as detector is reported for the first time in this work. The biosensor is constructed by gold film sputtering deposition on a stainless steel disk electrode and co-immobilization of the enzymes malate dehydrogenase (MDH) and diaphorase (DP) together with the redox mediator tetrathiafulvalene (TTF) by means of dialysis membrane. The analytical performance of the biosensor was evaluated when it was used as amperometric detector in three different analytical methodologies: stirred solutions, semiautomatic FIA system and automatic bioanalyzer. The bienzyme biosensor exhibited great analytical performance in terms of sensitivity, selectivity and reproducibility of the measurements and its usefulness was demonstrated by analyzing wine reference materials with certified content of L-malic acid. The attractive analytical and operational characteristics demonstrated by the automatic bioanalyzer make it a promising simple, rapid and field-based tool for routine wine and fruit control.
本文首次报道了一种新型的以集成安培生物传感器作为检测器的L-苹果酸自动生物分析仪。该生物传感器通过在不锈钢圆盘电极上溅射沉积金膜，并借助透析膜将苹果酸脱氢酶（MDH）、黄递酶（DP）与氧化还原介质四硫富瓦烯（TTF）共同固定而构建。当该生物传感器用作三种不同分析方法（搅拌溶液、半自动流动注射分析系统和自动生物分析仪）的安培检测器时，对其分析性能进行了评估。该双酶生物传感器在测量的灵敏度、选择性和重现性方面表现出优异的分析性能，通过分析具有L-苹果酸认证含量的葡萄酒参考物质证明了其实用性。自动生物分析仪所展示的吸引人的分析和操作特性使其成为一种用于常规葡萄酒和水果检测的有前景的简单、快速且基于现场的工具。
