Department of Chemistry, State Key Lab of Molecular Engineering of Polymers and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
Department of Chemistry, State Key Lab of Molecular Engineering of Polymers and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
Talanta. 2016 Sep 1;158:254-261. doi: 10.1016/j.talanta.2016.05.054. Epub 2016 May 19.
One of the main problems of the nanoparticle dispersion state change (e.g. from dispersion to aggregation) based surface-enhanced Raman scattering (SERS) detection is that the dynamic process of such in-solution reactions is always uncontrollable. This leads to poor reproducibility from a narrow time window of all such strategies, and finally great difference between the data from the diverse methods, and even between various sample batches. To address such problem, a facile, rapid SERS quantification protocol has been developed relying on target induced nanoparticle self-assembly at oil/water interfaces for copper ions analysis. In response to copper, the core-molecule-shell (CMS) nanoparticles spontaneously migrate to the interface and are assembled into densely packed arrays generating strong plasmonic coupling, which enables stable, sensitive and selective Raman quantitation, as well as visual detection. Also, this strategy shows capability for determination of large scale samples as the products can be stable for at least three weeks, and has been successfully applied to real sample detection. The developed Target Induced Nanoparticle Self-Assembled Interface (TINSAI) can be employed to both visual test and Raman quantitative detection, which would provide a platform for on-site screening as well as high-throughput detection with high sensitivity and selectivity.
基于表面增强拉曼散射（SERS）检测的纳米颗粒分散状态变化（例如从分散体到聚集体）的主要问题之一是，此类溶液反应的动态过程始终难以控制。这导致所有此类策略的时间窗口很窄，重现性较差，最终导致不同方法的数据之间存在很大差异，甚至不同样品批次之间也存在很大差异。为了解决这个问题，我们开发了一种简便、快速的 SERS 定量分析方法，该方法依赖于目标诱导的纳米颗粒在油水界面处的自组装，用于分析铜离子。对于铜离子，核-壳（CMS）纳米颗粒会自发迁移到界面，并组装成密集排列的阵列，产生强烈的等离子体耦合，从而实现稳定、灵敏和选择性的拉曼定量分析，以及可视化检测。此外，该策略还表现出能够用于大规模样品的测定能力，因为产物至少可以稳定 3 周，并且已经成功地应用于实际样品的检测。所开发的目标诱导纳米颗粒自组装界面（TINSAI）可用于可视化测试和拉曼定量检测，为现场筛选以及具有高灵敏度和选择性的高通量检测提供了一个平台。
