Allahyar Razia, Akbar Atif, Iqbal Furhan
1Institute of Pure and Applied Biology,Zoology Division,Bahauddin Zakariya University,Multan,Pakistan.
2Department of Statistics,Bahauddin Zakariya University,Multan,Pakistan.
Acta Neuropsychiatr. 2017 Feb;29(1):27-34. doi: 10.1017/neu.2016.28. Epub 2016 Jun 27.
Research findings made over the last few years have highlighted the important role of creatine (Cr) in health and disease. However, limited information is available regarding the effect of Cr supplementation on cognation. Present study was designed to determine the effect of variable doses of Cr (1% and 3%) on selected parameters of female albino mice behaviour.
Following weaning, on 20th postnatal day, female albino mice were divided into three groups on the basis of dietary supplementation. Control group were was fed with normal rodent diet, whereas treated groups received diet supplemented with 1% and 3% Creatine monohydrate (Ssniff, Germany) for 10 weeks. Morris water maze (MWM), Rota rod and open field (OF) tests were carried out at the end of diet supplementation for neurofunctional assessment in all the groups.
Data analysis showed that Cr supplementation did not affect the muscular activity and during rota rod test as well as locomotor and exploratory behaviour during OF test. Results of MWM probe trial indicated that mice supplemented with 3% Cr had significantly more entries in platform area than other two treatments (p=0.03) indicating improved spatial memory. Body weight remained unaffected (p>0.05) when compared between three experimental treatments.
Female mice supplemented with 3% Cr showed improved spatial memory than mice fed on 1% Cr-supplemented diet and mice on normal rodent diet.
过去几年的研究结果突出了肌酸（Cr）在健康和疾病中的重要作用。然而，关于补充肌酸对认知的影响，现有信息有限。本研究旨在确定不同剂量的肌酸（1%和3%）对雌性白化小鼠行为选定参数的影响。
断奶后，在出生后第20天，根据饮食补充情况将雌性白化小鼠分为三组。对照组喂食正常啮齿动物饮食，而处理组接受补充有1%和3%一水肌酸（德国Ssniff公司）的饮食，持续10周。在饮食补充结束时，对所有组进行莫里斯水迷宫（MWM）、转棒试验和旷场试验（OF），以进行神经功能评估。
数据分析表明，补充肌酸不影响转棒试验中的肌肉活动以及旷场试验中的运动和探索行为。MWM探针试验结果表明，补充3%肌酸的小鼠在平台区域的进入次数明显多于其他两种处理（p=0.03），表明空间记忆得到改善。三种实验处理之间比较时，体重未受影响（p>0.05）。
与喂食1%肌酸补充饮食的小鼠和正常啮齿动物饮食的小鼠相比，补充3%肌酸的雌性小鼠表现出更好的空间记忆。
