Institute of Pure and Applied Biology, Zoology Division, Bahauddin Zakariya University, Multan, Pakistan.
Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, Pakistan.
Naunyn Schmiedebergs Arch Pharmacol. 2020 Apr;393(4):717-725. doi: 10.1007/s00210-019-01787-z. Epub 2019 Dec 13.
Present study was conducted to report the effect of variable doses of neodymium zirconate zinc sulfide nanocomposite on behavior of albino mice of both sexes. Five-week-old albino mice (C57BL/6 strain) of both sexes were orally treated either with 10 mg (low dose) or 20 mg/ml saline/kg body weight (high dose) of neodymium zirconate zinc sulfide nanocomposite for 11 days. An untreated control group was maintained in parallel for same duration that received saline solution orally. A series of neurological (rotarod, light and dark box, open field, and novel object recognition) tests were conducted in all treatments. Oral supplementation of both low and high dose of nanocomposite significantly reduced the rotarod test performance as well as stretch attend reflex in male mice during light dark box test. Male mice treated with high dose of neodymium zirconate zinc sulfide nanocomposite had significantly increased time mobile and decreased time immobile than control group during open field test. Female mice treated with 10 mg/ml saline/kg body weight of neodymium zirconate zinc sulfide nanocomposite had significantly more line crossing during trial 1, and they spend more time with object A during trial 2 of novel object recognition test than their saline-treated control group. Change in body weight remained unaffected when compared between nanocomposite treated and untreated albino mice. In conclusion, we are reporting that both the applied doses of neodymium zirconate zinc sulfide nanocomposite are drastically affecting the muscular activity and exploratory behavior in male albino mice, while the studied behavioral tests, in general, remained unaffected in female albino mice.
本研究旨在报告不同剂量的钇锆锌硫化铈纳米复合材料对雌雄白化小鼠行为的影响。将 5 周龄的雌雄白化小鼠（C57BL/6 品系）分别用 10mg（低剂量）或 20mg/ml 生理盐水/kg 体重（高剂量）的钇锆锌硫化铈纳米复合材料经口处理 11 天。同时，维持一个未处理的对照组，给予相同剂量的生理盐水。所有处理组均进行了一系列神经学（转棒、明暗箱、旷场和新物体识别）测试。低剂量和高剂量纳米复合材料的经口补充均显著降低了雄性小鼠在明暗箱测试中的转棒测试表现和伸展反射。高剂量钇锆锌硫化铈纳米复合材料处理的雄性小鼠在旷场测试中比对照组表现出明显增加的移动时间和减少的静止时间。用 10mg/ml 生理盐水/kg 体重的钇锆锌硫化铈纳米复合材料处理的雌性小鼠在第 1 次试验中明显有更多的穿越线，在第 2 次新物体识别试验中，它们与物体 A 相处的时间比生理盐水处理的对照组更多。与未处理的白化小鼠相比，纳米复合材料处理组和未处理组的体重变化没有差异。总之，我们报告说，应用的钇锆锌硫化铈纳米复合材料剂量都严重影响雄性白化小鼠的肌肉活动和探索行为，而一般来说，研究中的行为测试在雌性白化小鼠中不受影响。
