Gao Guifang, Zhang Xiao-Fei, Hubbell Karen, Cui Xiaofeng
School of Chemistry, Chemical Engineering and Life Sciences, Wuhan University of Technology, 122 Luoshi Road, Wuhan, China.
Stemorgan Therapeutics, New York, New York.
Biotechnol Bioeng. 2017 Jan;114(1):208-216. doi: 10.1002/bit.26042. Epub 2016 Jul 19.
Bioprinting as an advanced enabling technology has the capacity to construct tissues with respective anatomical structures. In order to maintain the precise printing resolution for anatomical tissue printing, cell seeding density in bioink is limited. Bone marrow derived mesenchymal stem cells (MSCs) are widely used for cartilage tissue engineering. However, the approach of ideal chondrogenic differentiation of MSCs without hypertrophy still remains elusive. Here, we reported NR2F2 plays a crucial role in MSC chondrogenesis in bioprinted cartilage. NR2F2 over-expressed MSCs showed significantly enhanced chondrogenesis and NR2F2 knockdown cells demonstrated the exactly opposite behavior. We evaluated the cells cultured in monolayer, 3D pellet, and bioprinted 3D scaffold. All observations were consistent among gene expression, biochemical analysis, histological assay, and biomechanical evaluation. The data also revealed possible involvement of NR2F2 in mechanism of MSC chondrogenic differentiation under hypoxic culture condition. Biotechnol. Bioeng. 2017;114: 208-216. © 2016 Wiley Periodicals, Inc.
生物打印作为一种先进的赋能技术，有能力构建具有各自解剖结构的组织。为了在解剖组织打印中保持精确的打印分辨率，生物墨水的细胞接种密度受到限制。骨髓间充质干细胞（MSCs）被广泛用于软骨组织工程。然而，在不发生肥大的情况下使MSCs实现理想软骨分化的方法仍然难以捉摸。在此，我们报道了NR2F2在生物打印软骨中MSCs软骨形成过程中起着关键作用。过表达NR2F2的MSCs显示出显著增强的软骨形成能力，而敲低NR2F2的细胞则表现出完全相反的行为。我们评估了单层培养、三维微球培养以及生物打印三维支架培养的细胞。在基因表达、生化分析、组织学检测和生物力学评估方面，所有观察结果均一致。数据还揭示了NR2F2在低氧培养条件下MSCs软骨分化机制中的可能作用。《生物技术与生物工程》2017年；114卷：208 - 216页。© 2016威利期刊公司
