由剖宫产瘢痕来源的成纤维细胞产生的无转基因人诱导多能干细胞系（HS5-SV.hiPS）
Transgene-free human induced pluripotent stem cell line (HS5-SV.hiPS) generated from cesarean scar-derived fibroblasts.
作者信息
Rungsiwiwut Ruttachuk, Pavarajarn Wipawee, Numchaisrika Pranee, Virutamasen Pramuan, Pruksananonda Kamthorn
机构信息
Human Embryonic Stem Cell Research Center, Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Chulalongkorn University, Bangkok, Thailand.
Human Embryonic Stem Cell Research Center, Reproductive Medicine Unit, Department of Obstetrics and Gynecology, Chulalongkorn University, Bangkok, Thailand.
出版信息
Stem Cell Res. 2016 Jan;16(1):10-3. doi: 10.1016/j.scr.2015.11.006. Epub 2015 Nov 27.
Transgene-free human HS5-SV.hiPS line was generated from human cesarean scar-derived fibroblasts using temperature-sensitive Sendai virus vectors carrying Oct4, Sox2, cMyc and Klf4 exogenous transcriptional factors. The viral constructs were eliminated from HS5-SV.hiPS line through heat treatment. Transgene-free HS5-SV.hiPS cells expressed pluripotent associated transcription factors Oct4, Nanog, Sox2, Rex1 and surface markers SSEA-4, TRA-1-60 and OCT4. HS5-SV.hiPS cells formed embryoid bodies and differentiated into three embryonic germ layers in vivo. HS5-SV.hiPS cells maintained their normal karyotype (46, XX) after culture for extended period. HS5-SV.hiPS displayed the similar pattern of DNA fingerprinting to the parenteral scar-derived fibroblasts.
利用携带Oct4、Sox2、cMyc和Klf4外源性转录因子的温度敏感型仙台病毒载体，从人剖宫产瘢痕来源的成纤维细胞中生成了无转基因的人HS5-SV.hiPS细胞系。通过热处理从HS5-SV.hiPS细胞系中去除病毒构建体。无转基因的HS5-SV.hiPS细胞表达多能性相关转录因子Oct4、Nanog、Sox2、Rex1以及表面标志物SSEA-4、TRA-1-60和OCT4。HS5-SV.hiPS细胞形成胚状体并在体内分化为三个胚层。长时间培养后，HS5-SV.hiPS细胞维持其正常核型（46, XX）。HS5-SV.hiPS显示出与亲本瘢痕来源的成纤维细胞相似的DNA指纹图谱模式。
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