基于游离型载体从人胎儿包皮成纤维细胞生成诱导多能干细胞系
Episomal-based generation of an iPS cell line from human fetal foreskin fibroblasts.
作者信息
Matz Peggy, Adjaye James
机构信息
Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany; Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany; Institute of Biology, Humboldt University, 10099 Berlin, Germany.
Institute for Stem Cell Research and Regenerative Medicine, Medical Faculty, Heinrich Heine University, 40225 Düsseldorf, Germany; Max Planck Institute for Molecular Genetics, 14195 Berlin, Germany.
出版信息
Stem Cell Res. 2016 Jan;16(1):67-9. doi: 10.1016/j.scr.2015.12.009. Epub 2015 Dec 17.
Human fetal foreskin fibroblasts (HFF1) were used to generate the iPSC line epiHFF1-B1 employing a combination of three episomal-based plasmids expressing OCT4, SOX2, NANOG, LIN28, c-MYC, and KLF4. Pluripotency was confirmed both in vivo and in vitro. The transcriptome profile of epiHFF1-B1 and the human embryonic stem cell line-H1 have a pearson correlation of 0.936.
人胎儿包皮成纤维细胞（HFF1）被用于通过使用三种基于游离型质粒的组合来生成诱导多能干细胞系epiHFF1-B1，这些质粒表达OCT4、SOX2、NANOG、LIN28、c-MYC和KLF4。多能性在体内和体外均得到证实。epiHFF1-B1与人类胚胎干细胞系-H1的转录组图谱的皮尔逊相关系数为0.936。
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