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KCL034临床级人胚胎干细胞系的生成。

Generation of KCL034 clinical grade human embryonic stem cell line.

作者信息

Devito Liani, Jacquet Laureen, Petrova Anastasia, Miere Cristian, Wood Victoria, Kadeva Neli, Cornwell Glenda, Codognotto Stefano, Stephenson Emma, Ilic Dusko

机构信息

Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.

Stem Cell Laboratories, Division of Women's Health, Faculty of Life Sciences and Medicine, King's College London and Assisted Conception Unit, Guys' Hospital, London, United Kingdom.

出版信息

Stem Cell Res. 2016 Jan;16(1):184-8. doi: 10.1016/j.scr.2015.12.034. Epub 2016 Jan 3.

DOI:10.1016/j.scr.2015.12.034
PMID:27345810
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4757774/
Abstract

The KCL034 human embryonic stem cell line was derived from a normal healthy blastocyst donated for research. The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment and under current Good Manufacturing Practice (cGMP) standards. Pluripotent state and differentiation potential were confirmed by in vitro assays. The line was also validated for sterility, specific and non-specific human pathogens.

摘要

KCL034人胚胎干细胞系源自一个为研究而捐赠的正常健康囊胚。使用激光显微手术分离内细胞团，并接种于经γ射线照射的人包皮成纤维细胞上。干细胞的分离和细胞系的扩增均在无动物产品的环境中并按照现行药品生产质量管理规范（cGMP）标准进行。通过体外试验确认了其多能状态和分化潜能。该细胞系还经过了无菌、特定和非特定人类病原体的验证。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21c9/4757774/9317cca08019/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21c9/4757774/add002c334f6/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21c9/4757774/35e386313004/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21c9/4757774/7ee35b7b0306/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21c9/4757774/9317cca08019/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21c9/4757774/add002c334f6/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21c9/4757774/35e386313004/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21c9/4757774/7ee35b7b0306/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21c9/4757774/9317cca08019/gr4.jpg

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本文引用的文献

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The Molecular Karyotype of 25 Clinical-Grade Human Embryonic Stem Cell Lines.25种临床级人胚胎干细胞系的分子核型
Sci Rep. 2015 Nov 26;5:17258. doi: 10.1038/srep17258.
2
Three Huntington's Disease Specific Mutation-Carrying Human Embryonic Stem Cell Lines Have Stable Number of CAG Repeats upon In Vitro Differentiation into Cardiomyocytes.三种携带亨廷顿舞蹈症特定突变的人类胚胎干细胞系在体外分化为心肌细胞时，CAG重复序列数量稳定。
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A thyroid hormone receptor/KLF9 axis in human hepatocytes and pluripotent stem cells.
人类肝细胞和多能干细胞中的甲状腺激素受体/KLF9轴
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Cost-effective master cell bank validation of multiple clinical-grade human pluripotent stem cell lines from a single donor.来自单一供体的多种临床级人多能干细胞系的经济高效的主细胞库验证。
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3D In vitro model of a functional epidermal permeability barrier from human embryonic stem cells and induced pluripotent stem cells.人胚胎干细胞和诱导多能干细胞构建的功能性表皮渗透屏障的 3D 体外模型。
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The Database of Genomic Variants: a curated collection of structural variation in the human genome.基因组变异数据库：人类基因组中结构变异的精心整理集合。
Nucleic Acids Res. 2014 Jan;42(Database issue):D986-92. doi: 10.1093/nar/gkt958. Epub 2013 Oct 29.
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Strategy for the creation of clinical grade hESC line banks that HLA-match a target population.创建与目标人群 HLA 匹配的临床级 hESC 系银行的策略。
EMBO Mol Med. 2013 Jan;5(1):10-7. doi: 10.1002/emmm.201201973. Epub 2012 Nov 19.
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Derivation and propagation of human embryonic stem cell lines from frozen embryos in an animal product-free environment.在无动物产品环境中从冷冻胚胎中衍生和增殖人类胚胎干细胞系。
Nat Protoc. 2012 Jun 21;7(7):1366-81. doi: 10.1038/nprot.2012.080.
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Self-renewing endodermal progenitor lines generated from human pluripotent stem cells.人多能干细胞来源的自我更新内胚层祖细胞系。
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Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions.无饲养层且无异源条件下人类胚胎干细胞的分离和扩增。
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