Yang Honggai, Qu Zhuo, Zhang Jingze, Huo Liqin, Gao Jing, Gao Wenyuan
a School of Pharmaceutical Science and Technology , Tianjin University , Tianjin , China ;
b Department of Pharmacy , Logistics College of Chinese People's Armed Police Forces , Tianjin , China.
Int J Food Sci Nutr. 2016 Nov;67(7):806-17. doi: 10.1080/09637486.2016.1198890. Epub 2016 Jun 26.
Ferulic acid (FA) acts as a powerful antioxidant against various age-related diseases. To investigate the effect and underlying mechanism of FA against d-galactose(d-gal)-induced memory deficit, mice were injected with d-gal to induce memory impairment and simultaneously treated with FA and donepezil. The behavioral results revealed that chronic FA treatment reversed d-gal-induced memory impairment. Further, FA treatment inhibited d-gal-induced AChE activity and oxidative stress via increase of superoxide dismutase activity and reduced glutathione content, as well as decrease of malondialdehyde and nitric oxide levels. We also observed that FA significantly inhibits inflammation in the brain through reduction of NF-κB and IL-1β by enzyme-linked immunosorbent assay. Additionally, FA treatment significantly reduces the caspase-3 level in the hippocampus of d-gal-treated mice. Hematoxylin and eosin and Nissl staining showed that FA prevents neurodegeneration induced by d-gal. These findings showed that FA inhibits d-gal-induced AChE activity, oxidative stress, neuroinflammation and neurodegeneration, and consequently ameliorates memory impairment.
阿魏酸（FA）作为一种强大的抗氧化剂，可抵御各种与年龄相关的疾病。为了研究FA对d-半乳糖（d-gal）诱导的记忆缺陷的影响及潜在机制，给小鼠注射d-gal以诱导记忆损伤，同时用FA和多奈哌齐进行治疗。行为学结果显示，长期FA治疗可逆转d-gal诱导的记忆损伤。此外，FA治疗通过提高超氧化物歧化酶活性和降低谷胱甘肽含量，以及降低丙二醛和一氧化氮水平，抑制了d-gal诱导的乙酰胆碱酯酶（AChE）活性和氧化应激。我们还观察到，通过酶联免疫吸附测定法，FA可通过降低核因子κB（NF-κB）和白细胞介素-1β（IL-1β）显著抑制大脑炎症。此外，FA治疗可显著降低d-gal处理小鼠海马体中的半胱天冬酶-3水平。苏木精-伊红染色和尼氏染色显示，FA可预防d-gal诱导的神经退行性变。这些发现表明，FA可抑制d-gal诱导的AChE活性、氧化应激、神经炎症和神经退行性变，从而改善记忆损伤。
