表达红色荧光蛋白的人类胚胎干细胞系的建立：BJNhem20-pCAG-tdTomato
Generation of human embryonic stem cell line expressing a red fluorescent protein: BJNhem20-pCAG-tdTomato.
作者信息
Shetty Deeti K, Inamdar Maneesha S
机构信息
Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India.
Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India; Institute for Stem Cell Biology and Regenerative Medicine, Bengaluru, India.
出版信息
Stem Cell Res. 2016 Mar;16(2):290-2. doi: 10.1016/j.scr.2015.12.046. Epub 2016 Jan 6.
Human embryonic stem cell line BJNhem20-pCAG-tdTomato was generated using non-viral method. The construct pCAG-tdTomato was transfected using microporation procedure. This fluorescent hESC line can help to study heterogeneity within individual cells in hESC colonies by enabling live tracking of their growth, migration and differentiation properties. This cell line also serves as a resource for additional transgene introduction/knock-out/knock-in generation in a fluorescent background and allows ease of analysis in studies involving cell mixing.
人类胚胎干细胞系BJNhem20-pCAG-tdTomato是采用非病毒方法构建的。使用微穿孔程序转染构建体pCAG-tdTomato。这种荧光人类胚胎干细胞系能够实时追踪细胞的生长、迁移和分化特性，有助于研究人类胚胎干细胞集落中单个细胞的异质性。该细胞系还可作为一种资源，用于在荧光背景下进行额外的转基因导入/敲除/敲入操作，并便于在涉及细胞混合的研究中进行分析。
Zotero 插件