使用CRISPR/CAS9介导的靶向技术生成OCIAD1基因座的杂合敲除人类胚胎干细胞系：BJNhem20 - OCIAD1 - CRISPR - 39。
Generation of a heterozygous knockout human embryonic stem cell line for the OCIAD1 locus using CRISPR/CAS9 mediated targeting: BJNhem20-OCIAD1-CRISPR-39.
作者信息
Shetty Deeti K, Inamdar Maneesha S
机构信息
Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India.
Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India; Institute for Stem Cell Biology and Regenerative Medicine, Bengaluru, India.
出版信息
Stem Cell Res. 2016 Mar;16(2):308-10. doi: 10.1016/j.scr.2015.12.037. Epub 2016 Jan 5.
Ovarian carcinoma immuno-reactive antigen domain containing 1 (OCIAD1) single copy was knocked out generating an OCIAD1 heterozygous knockout human embryonic stem line named BJNhem20-OCIAD1-CRISPR-39. The line was generated using CRISPR-Cas9D10A double nickase knockout strategy (Mali et al., 2013).
敲除含1个拷贝的卵巢癌免疫反应抗原结构域1（OCIAD1），生成了一个名为BJNhem20 - OCIAD1 - CRISPR - 39的OCIAD1杂合敲除人类胚胎干细胞系。该细胞系是使用CRISPR - Cas9D10A双切口酶敲除策略生成的（Mali等人，2013年）。
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