Shetty Deeti K, Shetty Ronak, Inamdar Maneesha S
Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India.
Jawaharlal Nehru Centre for Advanced Scientific Research, Bengaluru, India; Institute for Stem Cell Biology and Regenerative Medicine, Bengaluru, India.
Stem Cell Res. 2016 Mar;16(2):246-8. doi: 10.1016/j.scr.2015.12.045. Epub 2016 Jan 7.
Human embryonic stem cell line BJNhem20-OCIAD1-Tet-On was generated using non-viral method. The constructs pCAG-Tet-On and pTRE-Tight vector driving OCIAD1 expression were transfected using microporation procedure. pCAG-Tet-On cells can be used for inducible expression of any coding sequence cloned into pTRE-Tight vector. For example, in human embryonic stem cells, Tet-On system has been used to generate SOX2 overexpression cell line (Adachi et al., 2010).
人类胚胎干细胞系BJNhem20 - OCIAD1 - Tet - On采用非病毒方法构建。使用微穿孔程序转染驱动OCIAD1表达的构建体pCAG - Tet - On和pTRE - Tight载体。pCAG - Tet - On细胞可用于诱导表达克隆到pTRE - Tight载体中的任何编码序列。例如，在人类胚胎干细胞中，Tet - On系统已被用于生成SOX2过表达细胞系（足立等人，2010年）。
