利用CRISPR/Cas9技术生成Nrf2纯合敲除的人类胚胎干细胞系。
Generation of a Nrf2 homozygous knockout human embryonic stem cell line using CRISPR/Cas9.
作者信息
Kim So-Jung, Habib Omer, Kim Jin-Soo, Han Hyo-Won, Koo Soo Kyung, Kim Jung-Hyun
机构信息
Division of Intractable Diseases, Center for Biomedical Sciences, Korea National Institute of Health, Cheongju 28159, Republic of Korea; Department of Program in Cell Biology and Genetics, College of Medicine, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Center for Genome Engineering, Institute for Basic Science (IBS), Seoul 08826, Republic of Korea; Department of Chemistry, Seoul National University, Seoul 08826, South Korea.
出版信息
Stem Cell Res. 2017 Mar;19:46-48. doi: 10.1016/j.scr.2016.12.027. Epub 2016 Dec 23.
Nuclear factor erythroid 2-related factor 2 (NFE2L2 or Nrf2) is a well-known transcription factor that regulates the expression of a large number of anti-oxidant genes in mammalian cells (J.H. Kim et al., 2014). Here, we generated a homozygous Nrf2 knockout human embryonic stem cell (hESC) line, H9Nrf2KO-A13, using the CRISPR/Cas9 genome editing method. The Nrf2 homozygous knockout H9 cell line maintains pluripotency, differentiation potential into three germ layers, and a normal karyotype.
核因子红细胞2相关因子2（NFE2L2或Nrf2）是一种著名的转录因子，可调节哺乳动物细胞中大量抗氧化基因的表达（J.H. Kim等人，2014年）。在此，我们使用CRISPR/Cas9基因组编辑方法生成了纯合Nrf2敲除人类胚胎干细胞（hESC）系H9Nrf2KO-A13。Nrf2纯合敲除H9细胞系保持多能性、分化为三个胚层的潜能以及正常的核型。
Zotero 插件