Sun Lan, Zhang Huaibao, Bi Laixi, Shi Yi-Fen, Xing Chongyun, Tang Liyuan, Jiang Songfu, Yu Kang
Department of Hematology, The First Affiliated Hospital of Wenzhou Medical University NanBai Xiang, Ouhai District, Wenzhou 325000, China.
Department of Orthopaedics, The First Affiliated Hospital of Wenzhou Medical University NanBai Xiang, Ouhai District, Wenzhou 325000, China.
Am J Transl Res. 2016 May 15;8(5):2011-21. eCollection 2016.
Angiopoietin-1 (Ang-1) plays a critical role in the regulation of endothelial cell survival and vascular maturation and stability. However, its role in hematopoiesis is not clear. Here, we determined effect of Ang-1 on the recovery of hematopoiesis in radiated mice. By injecting an Ang-1 plasmid, we found that Ang-1 was preferentially expressed in bone marrow (BM) of femur from radiated mice. This injection resulted in elevated blood counts and serum VEGF level. The blockade in S phase of cell cycle in mouse BM stromal cells following radiation was attenuated by injection of Ang-1 plasmid. In addition, injection of Ang-1 plasmid attenuated the radiation-mediated inhibition of Tie2 expression. Furthermore, through analyzing Notch1 expression, we found that injection of Ang-1 plasmid increased Notch mRNA expression in radiated mice. In conclusion, these findings suggest that Ang-1 facilitates the recovery of hematopoiesis in radiated mice with the involvement of Notch signaling pathway.
血管生成素-1（Ang-1）在调节内皮细胞存活、血管成熟和稳定性方面发挥着关键作用。然而，其在造血过程中的作用尚不清楚。在此，我们确定了Ang-1对辐射小鼠造血恢复的影响。通过注射Ang-1质粒，我们发现Ang-1在辐射小鼠股骨的骨髓（BM）中优先表达。这种注射导致血细胞计数和血清VEGF水平升高。注射Ang-1质粒减弱了辐射后小鼠骨髓基质细胞细胞周期S期的阻滞。此外，注射Ang-1质粒减弱了辐射介导的Tie2表达抑制。此外，通过分析Notch1表达，我们发现注射Ang-1质粒增加了辐射小鼠中Notch mRNA的表达。总之，这些发现表明，Ang-1通过Notch信号通路促进辐射小鼠的造血恢复。
