Yu Rui, Fang Ting, Liu Shuling, Song Xiaohong, Yu Changming, Li Jianmin, Fu Ling, Hou Lihua, Xu Junjie, Chen Wei
Laboratory of Vaccine and Antibody Engineering, Beijing Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijing 100071, China.
Toxins (Basel). 2016 Jun 25;8(7):194. doi: 10.3390/toxins8070194.
Tetanus is caused by the tetanus neurotoxin (TeNT) and is one of the most dreaded diseases especially in the developing countries. The current vaccine against tetanus is based on an inactivated tetanus toxin, which is effective but has many drawbacks. In our previous study, we developed a recombinant tetanus vaccine based on protein TeNT-Hc, with clear advantages over the toxoid vaccine in terms of production, characterization, and homogeneity. In this study, the titers, growth extinction, and persistence of specific antibodies induced by the two types of vaccine in mice, rats, and cynomolgus monkeys were compared. The booster vaccination efficacy of the two types of vaccines at different time points and protection mechanism in animals were also compared. The recombinant tetanus vaccine induced persistent and better antibody titers and strengthened the immunity compared with the commercially available toxoid vaccine in animals. Our results provide a theoretical basis for the development of a safe and effective recombinant tetanus vaccine to enhance the immunity of adolescents and adults as a substitute for the current toxoid vaccine.
破伤风由破伤风神经毒素（TeNT）引起，是最可怕的疾病之一，在发展中国家尤为如此。目前的破伤风疫苗基于灭活破伤风毒素，虽有效但存在诸多缺点。在我们之前的研究中，我们开发了一种基于蛋白TeNT-Hc的重组破伤风疫苗，在生产、特性及均一性方面比类毒素疫苗具有明显优势。在本研究中，比较了两种疫苗在小鼠、大鼠和食蟹猴体内诱导产生的特异性抗体的滴度、生长消退情况及持久性。还比较了两种疫苗在不同时间点的加强免疫效果及在动物体内的保护机制。与市售类毒素疫苗相比，重组破伤风疫苗在动物体内诱导产生了持续且更高的抗体滴度，并增强了免疫力。我们的结果为开发一种安全有效的重组破伤风疫苗以增强青少年和成人的免疫力作为当前类毒素疫苗的替代品提供了理论依据。
