Department of Chemistry and A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119992, Russia.
Antibiotics (Basel). 2016 Jun 24;5(3):22. doi: 10.3390/antibiotics5030022.
The machinery of translation is one of the most common targets of antibiotics. The development and screening of new antibiotics usually proceeds by testing antimicrobial activity followed by laborious studies of the mechanism of action. High-throughput methods for new antibiotic screening based on antimicrobial activity have become routine; however, identification of molecular targets is usually a challenge. Therefore, it is highly beneficial to combine primary screening with the identification of the mechanism of action. In this review, we describe a collection of methods for screening translation inhibitors, with a special emphasis on methods which can be performed in a high-throughput manner.
翻译机器是抗生素最常见的靶标之一。新抗生素的开发和筛选通常通过测试抗菌活性，然后进行艰苦的作用机制研究来进行。基于抗菌活性的新抗生素筛选的高通量方法已成为常规；然而，分子靶标的鉴定通常是一个挑战。因此，将初步筛选与作用机制的鉴定相结合是非常有益的。在这篇综述中，我们描述了一系列筛选翻译抑制剂的方法，特别强调了可以高通量进行的方法。
