Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Key Laboratory of Sensor Analysis of Tumor Marker, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
Biosens Bioelectron. 2016 Dec 15;86:143-149. doi: 10.1016/j.bios.2016.06.028. Epub 2016 Jun 11.
An ultrasensitive biosensor for alpha-fetoprotein was developed based on electrochemically synthesized polyaniline (PANI) nanowires, which were functionalized with gold nanoparticles (AuNPs) and polyethylene glycols (PEG). The prepared PEG/AuNPs/PANI composite, combining the electrical conductivity of the AuNPs/PANI with the robust antifouling ability of PEG, offered an ideal substrate for the development of low fouling electrochemical biosensors. Alpha-fetoprotein (AFP), a well-known hepatocellular carcinoma biomarker, was used as a model analyte, and its antibody was immobilized on the PEG/AuNPs/PANI for the construction of the AFP immunosensor. Using the redox current of PANI as the sensing signal, in addition to the good biocompatibility of PEG/AuNPs and the anti-biofouling property of PEG, the developed immunosensor showed improved biosensing performances, such as wide linear range and ultralow detection limit (0.007pgmL(-1)). More importantly, it is label-free, reagentless and low fouling, making it capable of assaying AFP in real serum samples without suffering from significant interference or biofouling.
基于电化学合成的聚苯胺（PANI）纳米线，研制出一种用于甲胎蛋白的超灵敏生物传感器，该纳米线功能化了金纳米粒子（AuNPs）和聚乙二醇（PEG）。所制备的 PEG/AuNPs/PANI 复合材料结合了 AuNPs/PANI 的导电性和 PEG 的强抗污能力，为开发低污染电化学生物传感器提供了理想的基底。甲胎蛋白（AFP）是一种著名的肝癌生物标志物，被用作模型分析物，其抗体被固定在 PEG/AuNPs/PANI 上，用于构建 AFP 免疫传感器。利用 PANI 的氧化还原电流作为传感信号，除了 PEG/AuNPs 的良好生物相容性和 PEG 的抗污性能外，所开发的免疫传感器还表现出改进的生物传感性能，如较宽的线性范围和超低的检测限（0.007pgmL(-1)）。更重要的是，它是无标记、无试剂且低污染的，能够在真实血清样本中测定 AFP，而不会受到显著干扰或污染。
