Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China.
Sci Rep. 2016 Jun 28;6:28678. doi: 10.1038/srep28678.
Small interfering RNAs (siRNAs) and microRNAs (miRNAs) are two classes of small RNAs (sRNAs) that are critical for virus-host interplay via the RNA interference (RNAi) pathway. One virus-derived siRNA and numerous miRNAs has been reported for the double-stranded DNA virus white spot syndrome virus (WSSV), however, the expression profiles of these different types of sRNAs have not been assessed. Here, by sequencing the sRNAs and mRNAs of WSSV-infected Chinese shrimp (Fenneropenaeus chinensis), we found that the viral transcripts were universally targeted by WSSV-derived siRNAs, supporting a pivotal role for RNAi in the anti-viral immunity of shrimp. The genesis of WSSV-derived siRNAs was associated with long RNA structures. Moreover, by separating miRNAs from siRNAs, 12 WSSV miRNAs were identified. Investigation of conserved viral miRNA targets in different host species indicated the involvement of viral miRNAs in host immune responses. Collectively, our data provide new insights into the role of the RNAi pathway in the interplay between DNA viruses and crustaceans.
小干扰 RNA（siRNAs）和 microRNA（miRNAs）是两类小 RNA（sRNAs），通过 RNA 干扰（RNAi）途径，对病毒-宿主相互作用至关重要。已报道双链 DNA 病毒白斑综合征病毒（WSSV）有一个病毒来源的 siRNA 和许多 miRNAs，但这些不同类型的 sRNAs 的表达谱尚未评估。在这里，通过对 WSSV 感染的中国对虾（Fenneropenaeus chinensis）的 sRNA 和 mRNA 进行测序，我们发现病毒转录物普遍受到 WSSV 来源的 siRNAs 的靶向，这支持了 RNAi 在虾抗病毒免疫中的关键作用。WSSV 来源的 siRNAs 的产生与长 RNA 结构有关。此外，通过将 miRNAs 与 siRNAs 分离，鉴定出 12 种 WSSV miRNAs。对不同宿主物种中保守的病毒 miRNA 靶标的研究表明，病毒 miRNA 参与了宿主的免疫反应。总的来说，我们的数据为 RNAi 途径在 DNA 病毒与甲壳动物相互作用中的作用提供了新的见解。
