小RNA应对大型病毒：昆虫中基于RNA干扰的针对DNA病毒的抗病毒防御。
Small RNAs tackle large viruses: RNA interference-based antiviral defense against DNA viruses in insects.
作者信息
Bronkhorst Alfred W, Miesen Pascal, van Rij Ronald P
机构信息
Department of Medical Microbiology; Radboud University Nijmegen Medical Centre; Nijmegen Centre for Molecular Life Sciences; Nijmegen Institute for Infection, Inflammation and Immunity; Nijmegen, The Netherlands.
出版信息
Fly (Austin). 2013 Oct-Dec;7(4):216-23. doi: 10.4161/fly.25708. Epub 2013 Aug 23.
Abstract
The antiviral RNA interference (RNAi) pathway processes viral double-stranded RNA (dsRNA) into viral small interfering RNAs (vsiRNA) that guide the recognition and cleavage of complementary viral target RNAs. In RNA virus infections, viral replication intermediates, dsRNA genomes or viral structured RNAs have been implicated as Dicer-2 substrates. In a recent publication, we demonstrated that a double-stranded DNA virus, Invertebrate iridescent virus 6, is a target of the Drosophila RNAi machinery, and we proposed that overlapping converging transcripts base pair to form the dsRNA substrates for vsiRNA biogenesis. Here, we discuss the role of RNAi in antiviral defense to DNA viruses in Drosophila and other invertebrate model systems.
摘要
抗病毒RNA干扰（RNAi）途径将病毒双链RNA（dsRNA）加工成病毒小干扰RNA（vsiRNA），后者引导对互补病毒靶RNA的识别和切割。在RNA病毒感染中，病毒复制中间体、dsRNA基因组或病毒结构RNA被认为是Dicer-2的底物。在最近的一篇出版物中，我们证明了一种双链DNA病毒——无脊椎动物虹彩病毒6是果蝇RNAi机制的靶标，并且我们提出重叠的汇聚转录本通过碱基配对形成vsiRNA生物合成的dsRNA底物。在这里，我们讨论RNAi在果蝇和其他无脊椎动物模型系统中对DNA病毒的抗病毒防御中的作用。
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