Marques Lucia C F, Pinheiro Aruanã J M C R, Araújo João G G, de Oliveira Raimundo A G, Silva Selma N, Abreu Iracelle C, de Sousa Eduardo M, Fernandes Elizabeth S, Luchessi André D, Silbiger Vivian N, Nicolete Roberto, Lima-Neto Lidio G
Programa de Pós-Graduação, Universidade CEUMA, Sao Luis, Brazil.
Centro de Pesquisa Clínica, Universidade Federal do Maranhão, São Luis, Brazil.
Planta Med. 2016 Nov;82(17):1463-1467. doi: 10.1055/s-0042-108856. Epub 2016 Jun 28.
Folk medicine suggests that pomegranate (peels, seeds and leaves) has anti-inflammatory properties; however, the precise mechanisms by which this plant affects the inflammatory process remain unclear. Herein, we analyzed the anti-inflammatory properties of a hydroalcoholic extract prepared from pomegranate leaves using a rat model of lipopolysaccharide-induced acute peritonitis. Male Wistar rats were treated with either the hydroalcoholic extract, sodium diclofenac, or saline, and 1 h later received an intraperitoneal injection of lipopolysaccharides. Saline-injected animals (i. p.) were used as controls. Animals were culled 4 h after peritonitis induction, and peritoneal lavage and peripheral blood samples were collected. Serum and peritoneal lavage levels of TNF- as well as TNF- mRNA expression in peritoneal lavage leukocytes were quantified. Total and differential leukocyte populations were analyzed in peritoneal lavage samples. Lipopolysaccharide-induced increases of both TNF- mRNA and protein levels were diminished by treatment with either pomegranate leaf hydroalcoholic extract (57 % and 48 % mean reduction, respectively) or sodium diclofenac (41 % and 33 % reduction, respectively). Additionally, the numbers of peritoneal leukocytes, especially neutrophils, were markedly reduced in hydroalcoholic extract-treated rats with acute peritonitis. These results demonstrate that pomegranate leaf extract may be used as an anti-inflammatory drug which suppresses the levels of TNF- in acute inflammation.
民间医学认为石榴（果皮、种子和叶子）具有抗炎特性；然而，这种植物影响炎症过程的确切机制仍不清楚。在此，我们使用脂多糖诱导的急性腹膜炎大鼠模型分析了石榴叶制备的水醇提取物的抗炎特性。将雄性Wistar大鼠分别用该水醇提取物、双氯芬酸钠或生理盐水处理，1小时后腹腔注射脂多糖。注射生理盐水的动物（腹腔注射）用作对照。在诱导腹膜炎4小时后处死动物，并收集腹腔灌洗和外周血样本。对血清和腹腔灌洗中的肿瘤坏死因子-α水平以及腹腔灌洗白细胞中的肿瘤坏死因子-α mRNA表达进行定量分析。对腹腔灌洗样本中的白细胞总数和分类白细胞群体进行分析。用石榴叶水醇提取物（平均降低分别为57%和48%）或双氯芬酸钠（分别降低41%和33%）处理可减轻脂多糖诱导的肿瘤坏死因子-α mRNA和蛋白质水平的升高。此外，在患有急性腹膜炎的水醇提取物处理的大鼠中，腹腔白细胞，尤其是中性粒细胞的数量明显减少。这些结果表明，石榴叶提取物可用作抗炎药物，在急性炎症中抑制肿瘤坏死因子-α的水平。
