Stope Matthias B, Wiegank Luise, Weiss Martin, Diesing Karoline, Koensgen Dominique, Burchardt Martin, Zygmunt Marek, Mustea Alexander
Department of Urology, University of Medicine Greifswald, Greifswald, Germany
Department of Obstetrics and Gynaecology, University of Medicine Greifswald, Greifswald, Germany.
Anticancer Res. 2016 Jul;36(7):3321-7.
Heat-shock protein HSPB1 (alternative name HSP27) plays a pivotal role in cell survival pathways, apoptosis, metastasis and has been frequently linked to treatment resistance in ovarian cancer (OC) and other malignancies. Characteristic HSPB1 induction in different solid tumors is often caused by cytotoxic agents.
An in vitro OC cell model system was established to characterize resistance mechanisms during chemotherapy. Human OC cell lines OVCAR-3, SK-OV-3 and TOV-21G were treated with paclitaxel or carboplatin. Cellular growth was analyzed by cell counting. Intra- and extracellular HSPB1 concentrations were assessed by western blot and enzyme-linked immunosorbent assays.
Incubation with paclitaxel, and with carboplatin significantly reduced cell growth without a definitive increase of intracellular HSPB1 expression. HSPB1 demonstrated drug-inducible secretion into the extracellular compartment.
Despite its current lack of analysis in patient samples, serum soluble HSPB1 may function as a specific biomarker for monitoring response to chemotherapy in patients with OC.
热休克蛋白HSPB1（别名HSP27）在细胞存活途径、凋亡、转移中起关键作用，并且常与卵巢癌（OC）及其他恶性肿瘤的治疗耐药性相关。不同实体瘤中HSPB1的特征性诱导通常由细胞毒性药物引起。
建立体外OC细胞模型系统以表征化疗期间的耐药机制。用紫杉醇或卡铂处理人OC细胞系OVCAR-3、SK-OV-3和TOV-21G。通过细胞计数分析细胞生长。通过蛋白质免疫印迹和酶联免疫吸附测定评估细胞内和细胞外HSPB1浓度。
用紫杉醇和卡铂孵育显著降低细胞生长，而细胞内HSPB1表达无明确增加。HSPB1表现出药物诱导的向细胞外区室的分泌。
尽管目前尚未对患者样本进行分析，但血清可溶性HSPB1可能作为监测OC患者化疗反应的特异性生物标志物。
