Lu Ren-quan, Sun Min, Gao Xiang, Guo Lin
Department of Clinical Laboratory, Shanghai Cancer Center, Shanghai Medical College, Fudan University, Shanghai 200032, China.
Zhonghua Zhong Liu Za Zhi. 2012 Mar;34(3):176-81. doi: 10.3760/cma.j.issn.0253-3766.2012.03.004.
To investigate the expression of a novel biomarker, human short-type myofibrillogenesis regulator-1 (MR-1S), in ovarian carcinoma and explore its biological significance.
The MR-1S mRNA levels were analyzed by reverse transcription polymerase chain reaction (RT-PCR) in 23 specimens of ovarian cancers and 20 specimens of control ovarian tissues. The expression of MR-1S in these specimens was detected by real-time PCR and immunohistochemical analysis. In addition, the expression of MR-1S in ovarian cancer SKOV3 cells was determined by immunocytochemisty. MR-1S mRNA and protein level of SKOV3 cells was compared in the two groups treated by carboplatin and paclitaxel at 24 h, 48 h and 72 h, respectively. Furthermore, the expression of MR-1S was analyzed in liner concentration range of the anti-cancer drug, and the potential relation between MR-1S expression and cell apoptosis rate was predicted.
The level of MR-1S mRNA was significantly higher in ovarian cancer tissues than those of control tissues by RT-PCR and Real-time PCR analysis. MR-1S protein was overexpressed in ovarian cancer tissues with a positive rate of 78.3% (18/23) than that in the control tissues (30.0%, P<0.05) through IHC analysis. The expression of MR-1S was markedly decreased by treatment with carboplatin and paclitaxel, and there was a direct correlation between MR-1S expression and apoptosis rate, especially in a liner concentration range of paclitaxel at 48 h.
MR-1S is highly expressed in ovarian cancer cells and tissues, and it may be a promising biomarker for diagnosis and a new target for ovarian cancer therapy.
研究一种新型生物标志物人短型肌纤生成调节因子-1（MR-1S）在卵巢癌中的表达，并探讨其生物学意义。
采用逆转录聚合酶链反应（RT-PCR）分析23例卵巢癌组织和20例对照卵巢组织中MR-1S mRNA水平。通过实时荧光定量PCR和免疫组织化学分析检测这些标本中MR-1S的表达。此外，采用免疫细胞化学法检测MR-1S在卵巢癌SKOV3细胞中的表达。分别比较顺铂和紫杉醇处理24 h、48 h和72 h后两组SKOV3细胞中MR-1S mRNA和蛋白水平。此外，分析抗癌药物线性浓度范围内MR-1S的表达，并预测MR-1S表达与细胞凋亡率之间的潜在关系。
RT-PCR和实时荧光定量PCR分析显示，卵巢癌组织中MR-1S mRNA水平显著高于对照组织。免疫组织化学分析显示，MR-1S蛋白 在卵巢癌组织中高表达，阳性率为78.3%（18/23），高于对照组织（30.0%，P<0.05）。顺铂和紫杉醇处理后，MR-1S的表达明显降低，且MR-1S表达与凋亡率之间存在直接相关性，尤其是在48 h紫杉醇线性浓度范围内。
MR-1S在卵巢癌细胞和组织中高表达，可能是一种有前景的诊断生物标志物和卵巢癌治疗的新靶点。
