肿瘤抑制性微小RNA-1对卵巢癌细胞增殖具有特异性抑制作用。
The Tumor Suppressor MicroRNA-1 Exhibits Restricted Inhibition of Proliferation of Ovarian Cancer Cells.
作者信息
Stope Matthias B, Hettenbach Daria, Kaul Anne, Paditz Madeleine, Diesing Karoline, Burchardt Martin, Zygmunt Marek, Mustea Alexander, Koensgen Dominique
机构信息
Department of Urology, University of Medicine Greifswald, Greifswald, Germany
Department of Gynecology and Obstetrics, University of Medicine Greifswald, Greifswald, Germany.
出版信息
Anticancer Res. 2016 Jul;36(7):3329-34.
BACKGROUND
MicroRNAs are able to control vital tumor biological processes, such as proliferation, tissue transformation and cell migration, as well as apoptosis. One of the micro RNAs, namely miR-1, has been classified as a tumor suppressor, however, preliminary data did not confirm this finding in ovarian cancer (OC) cells. This study examined the impact of miR-1 on OC cell growth.
MATERIALS AND METHODS
Recombinant miR-1 was overexpressed in human OC cell lines OVCAR-3, SK-OV-3, TOV-112D, and TOV-21G. Subsequently, cell growth was analyzed.
RESULTS
After transfection, 11- to 487-fold overexpression of miR-1 was detectable in the OC cells. However, no significant differences in proliferation compared to control cells were detected, neither in transiently nor in stably transfected cells.
CONCLUSION
In numerous cancer entities miR-1 is defined as an antiproliferative tumor suppressor. Notably, the present study demonstrated a loss of growth-inhibitory functionality of miR-1 by so far unknown mechanisms, suggesting dysregulated miR-1 signaling or effector cascades in OC cells.
背景
微小RNA能够控制重要的肿瘤生物学过程，如增殖、组织转化和细胞迁移，以及细胞凋亡。其中一种微小RNA，即miR-1，已被归类为肿瘤抑制因子，然而，初步数据并未在卵巢癌细胞（OC）中证实这一发现。本研究检测了miR-1对OC细胞生长的影响。
材料与方法
重组miR-1在人OC细胞系OVCAR-3、SK-OV-3、TOV-112D和TOV-21G中过表达。随后，分析细胞生长情况。
结果
转染后，在OC细胞中可检测到miR-1有11至487倍的过表达。然而，与对照细胞相比，无论是瞬时转染还是稳定转染的细胞，其增殖均未检测到显著差异。
结论
在众多癌症实体中，miR-1被定义为抗增殖肿瘤抑制因子。值得注意的是，本研究表明miR-1的生长抑制功能因迄今未知的机制而丧失，提示OC细胞中miR-1信号传导或效应级联失调。
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