miR-424-3p 抑制半乳糖凝集素-3 的表达并增强卵巢癌细胞对顺铂的敏感性。
MiR-424-3p suppresses galectin-3 expression and sensitizes ovarian cancer cells to cisplatin.
机构信息
Department of Biotechnology and Genetic Engineering, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, Katowice, Poland, Jedności 8 Street, 41-200, Sosnowiec, Poland.
出版信息
Arch Gynecol Obstet. 2019 Apr;299(4):1077-1087. doi: 10.1007/s00404-018-4999-7. Epub 2018 Dec 26.
PURPOSE
Assessment of miR-424-3p mimic capability to sensitize SK-OV-3 and TOV-21G ovarian cancer cells to cisplatin by decreasing the expression of galectin-3, which is an anti-apoptotic protein overexpressed in ovarian cancer and associated with resistance to chemotherapy.
METHODS
We performed a reverse transfection of miR-424-3p mimic into SK-OV-3 and TOV-21G ovarian cancer cells, followed by Real Time™ RT-PCR analysis of the expression of miR-424-3p and galectin-3 mRNA as well as ELISA assay for galectin-3 protein level. Next, we studied the viability (XTT assay), proliferation (EdU incorporation assay), and apoptosis (ELISA assay) of the both cell lines transfected with the mimic and treated with cisplatin.
RESULTS
We demonstrated that miR-424-3p mimic effectively transfects into SK-OV-3 and TOV-21G ovarian cancer cells in which it significantly suppresses the expression of galectin-3 at the protein level, but not at the mRNA level. Reverse transfection of both cell lines with the mimic, followed by treatment with cisplatin, resulted in a reduction in cell viability and proliferation as well as an increase in the induction of apoptosis.
CONCLUSIONS
MiR-424-3p mimic sensitizes SK-OV-3 and TOV-21G ovarian cancer cells to cisplatin by decreasing the expression of galectin-3.
目的
通过降低在卵巢癌中过表达且与化疗耐药相关的抗凋亡蛋白半乳糖凝集素-3（galectin-3）的表达，评估 miR-424-3p 模拟物使 SK-OV-3 和 TOV-21G 卵巢癌细胞对顺铂敏感的能力。
方法
我们将 miR-424-3p 模拟物进行反转录转染至 SK-OV-3 和 TOV-21G 卵巢癌细胞中，随后进行 miR-424-3p 和 galectin-3 mRNA 的实时 RT-PCR 分析以及 galectin-3 蛋白水平的 ELISA 检测。接下来，我们研究了转染模拟物并用顺铂处理的两种细胞系的活力（XTT 测定）、增殖（EdU 掺入测定）和凋亡（ELISA 测定）。
结果
我们证明了 miR-424-3p 模拟物可有效地转染至 SK-OV-3 和 TOV-21G 卵巢癌细胞中，在这两种细胞中，它可显著抑制 galectin-3 的蛋白表达，但不影响其 mRNA 表达。两种细胞系的模拟物反转录转染后用顺铂处理，导致细胞活力和增殖降低，凋亡诱导增加。
结论
miR-424-3p 模拟物通过降低 galectin-3 的表达使 SK-OV-3 和 TOV-21G 卵巢癌细胞对顺铂敏感。
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