基质金属肽酶-1基因分型、吸烟、饮酒和嚼槟榔对鼻咽癌易感性的影响

Contribution of Matrix Metallopeptidase-1 Genotypes, Smoking, Alcohol Drinking and Areca Chewing to Nasopharyngeal Carcinoma Susceptibility.

作者信息

Tsai Chia-Wen, Chang Wen-Shin, Gong Chi-Li, Shih Liang-Chun, Chen Liang-Yu, Lin En-Yuan, Li Hsin-Ting, Yen Shiou-Ting, Wu Cheng-Nan, Bau DA-Tian

机构信息

Department of Medical Laboratory Science and Biotechnology, Central Taiwan University of Science and Technology, Taichung, Taiwan, R.O.C. Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.

Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C. Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, R.O.C.

出版信息

Anticancer Res. 2016 Jul;36(7):3335-40.

PMID:27354591

Abstract

BACKGROUND/AIM: The up-regulation of matrix metalloproteinase-1 (MMP-1) has been demonstrated to be correlated with lymph node metastasis of nasopharyngeal carcinoma (NPC); however, the genotypic role of MMP-1 is not well understand. The present study aimed to assess the contribution of MMP-1 promoter -1607 genotypes, combined with environmental carcinogens, on the predisposition to NPC tumorigenesis.

MATERIALS AND METHODS

The MMP-1 promoter -1607 genotypes were examined for 352 age- and gender-matched controls and 176 NPC patients by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methodology.

RESULTS

We found that the MMP-1 promoter -1607 heterozygous 1G/2G and homozygous 1G/1G genotypes, were more and more prone to be associated with NPC risk (odds ratio (OR)=0.64 and 0.63, 95% confidence interval (CI)=0.43-1.03 and 0.36-0.96, p=0.0659 and 0.0932, respectively). In the dominant models, there was a significant association between the genotype of MMP-1 promoter -1607 and NPC risk (OR=0.64, 95% CI=0.43-0.91, p=0.0359). In addition, individuals carrying the 1G allele at MMP-1 promoter -1607 were less susceptible to NPC (OR=0.73; 95%CI=0.59 to 0.96, p=0.0418) after adjustment for age, gender, cigarette, alcohol and areca consumption.

CONCLUSION

The 1G/1G genotype of MMP-1 promoter -1607 may independently have a protective effect on NPC risk, without interaction with behavioral factors, including cigarette, alcohol and areca consumption.

摘要

背景/目的：基质金属蛋白酶-1（MMP-1）的上调已被证明与鼻咽癌（NPC）的淋巴结转移相关；然而，MMP-1的基因型作用尚未完全明确。本研究旨在评估MMP-1启动子-1607基因型与环境致癌物联合作用对NPC发生易感性的影响。

材料与方法

采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对352名年龄和性别匹配的对照者及176例NPC患者进行MMP-1启动子-1607基因型检测。

结果

我们发现MMP-1启动子-1607杂合子1G/2G和纯合子1G/1G基因型与NPC风险的相关性越来越高（优势比（OR）分别为0.64和0.63，95%置信区间（CI）为0.43 - 1.03和0.36 - 0.96，p值分别为0.0659和0.0932）。在显性模型中，MMP-1启动子-1607基因型与NPC风险之间存在显著关联（OR = 0.64，95%CI = 0.43 - 0.91，p = 0.0359）。此外，在对年龄、性别、吸烟、饮酒和槟榔消费进行调整后，MMP-1启动子-1607携带1G等位基因的个体患NPC的易感性较低（OR = 0.73；95%CI = 0.59至0.96，p = 0.0418）。