Fukui Y
Department of Biology, Tokyo Women's Medical College, Japan.
Anat Embryol (Berl). 1989;179(4):347-53. doi: 10.1007/BF00305061.
The uropygial gland is one of the epidermal derivatives in birds. In the beginning of the morphogenesis of the uropygial gland of the quail embryo, placode formation occurs: Epidermal basal cells remain cuboidal until day 7 of incubation. At day 8 they begin to elongate, become columnar, and develop placode-like structure at day 9. To examine the proliferative activity of the epidermal basal cells, we recorded positions of [3H]-thymidine-labelled nuclei in serial sections by autoradiography (ARG) using a digitizer and a microcomputer. The distribution pattern of labelled nuclei was reconstituted and redisplayed on the cathode ray tube (CRT) as two-dimensional computer graphics. Three-dimensional reconstitution of the same serial sections revealed that cuboidal basal cells in the presumptive uropygial placode became columnar. Unlike the feather and scale placodes, the uropygial placode possessed no resting period of DNA synthesis. The labelling index of the uropygial placode increased considerably compared with that of nonplacode epidermis. No dermal condensation was observed beneath the uropygial placode.
尾脂腺是鸟类的表皮衍生物之一。在鹌鹑胚胎尾脂腺形态发生的开始阶段，基板形成：直到孵化第7天，表皮基底细胞仍为立方形。在第8天，它们开始伸长，变为柱状，并在第9天形成基板样结构。为了检测表皮基底细胞的增殖活性，我们通过使用数字转换器和微型计算机的放射自显影术（ARG）在连续切片中记录了[3H] - 胸腺嘧啶核苷标记的细胞核的位置。标记细胞核的分布模式被重建，并作为二维计算机图形重新显示在阴极射线管（CRT）上。对相同连续切片的三维重建显示，假定尾脂腺基板中的立方形基底细胞变为柱状。与羽毛和鳞片基板不同，尾脂腺基板没有DNA合成的静止期。与非基板表皮相比，尾脂腺基板的标记指数显著增加。在尾脂腺基板下方未观察到真皮凝聚。
