Fukui Y
Department of Biology, Tokyo Women's Medical College, Shinjuku-ku, Japan.
Dev Growth Differ. 1997 Apr;39(2):157-66. doi: 10.1046/j.1440-169x.1997.t01-1-00004.x.
Formation of the uropygial papilla and glandular lumena was inhibited when the uropygial rudiment of a day 8 1/3 quail embryo was cultured for 2 days in a chemically defined medium in the presence of 50 ng/mL of epidermal growth factor (EGF). The epithelium of EGF-treated explants remained at the placode stage, or underwent minor invagination into the mesenchyme and became stratified like that of a 12- or 13-day-old embryo. EGF promoted cellular proliferation in the uropygial epithelium and the epidermis adjacent to the gland and it shortened the lag phase of proliferation and markedly stimulated epithelial DNA synthesis, detected immunocytochemically by labeling explants with 5-bromodeoxyuridine (BrdU). The maximal labeling index in EGF-treated uropygial epithelium was 55% higher than in the control. Electron microscopic observation revealed that the basal lamina had become irregular in the EGF-treated explants and that epithelial cytoplasmic processes penetrated through the basal lamina toward the mesenchyme. These same phenomena are observed in vivo when the glandular buds are formed during day 12-13. Some precocious changes occurred in the uropygial epithelium when the rudiment was cultured in the presence of EGF.
当将8 1/3日龄鹌鹑胚胎的尾脂腺原基在含有50 ng/mL表皮生长因子（EGF）的化学限定培养基中培养2天时，尾脂腺乳头和腺腔的形成受到抑制。经EGF处理的外植体上皮细胞停留在基板阶段，或向间充质发生轻微内陷，并变得像12或13日龄胚胎的上皮细胞那样分层。EGF促进了尾脂腺上皮细胞和腺体附近表皮细胞的增殖，缩短了增殖的延迟期，并通过用5-溴脱氧尿苷（BrdU）标记外植体进行免疫细胞化学检测，显著刺激了上皮细胞的DNA合成。经EGF处理的尾脂腺上皮细胞的最大标记指数比对照组高55%。电子显微镜观察显示，经EGF处理的外植体中基膜变得不规则，上皮细胞质突起穿过基膜伸向间充质。当在12至13日龄形成腺芽时，在体内也观察到了同样的现象。当原基在EGF存在的情况下培养时，尾脂腺上皮细胞发生了一些早熟变化。
