生物样品中阿霉素及其代谢物的快速定量测定。
Rapid quantitative determination of doxorubicin and its metabolites in biological samples.
作者信息
Sqalli A, Labat C, Oustrin J, Houin G, Coulais Y, Bugat R, Carton P
机构信息
Laboratoire de Biophysique et de Biomathématique, Faculté de Pharmacie, Toulouse.
出版信息
Ann Biol Clin (Paris). 1989;47(2):63-6.
A rapid sensitive and selective method is developed for the plasma analysis of doxorubicin and its metabolites, doxorubicinol and doxorubicinone with daunorubicin as the internal standard, by using high performance liquid chromatography (HPLC) with fluorescence detection and a "zorbax ODS" column. An eluent containing tetrahydrofuran and trietylamine afforded improved efficiency and resolution and was used to resolve the four anthracycline derivatives in a sole isocratic run. An example of the plasma levels obtained in a cancerous patient after two different administrations is shown.
开发了一种快速、灵敏且选择性高的方法，以柔红霉素为内标，采用带荧光检测的高效液相色谱（HPLC）和“Zorbax ODS”柱，用于血浆中多柔比星及其代谢物多柔比星醇和多柔比星酮的分析。含有四氢呋喃和三乙胺的洗脱液提高了效率和分离度，并用于在单一等度洗脱中分离这四种蒽环类衍生物衍生物分离度。给出了一名癌症患者在两次不同给药后获得的血浆水平示例水平示例。
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