褐变抑制剂对明党参悬浮细胞生长及次生代谢产物合成的影响

[Effects of Browning Inhibitors on Suspension Cells Growth and Secondary Metabolites Production in Changium smyrnioides].

作者信息

Zhou Ping, Xie Chen-qiong, Chen Jian-wei, Li Xiang

出版信息

Zhong Yao Cai. 2015 Nov;38(11):2250-4.

Abstract

OBJECTIVE

To study the effects of browning inhibitors on Changium smyrnioides suspension cells growth and secondary metabolites production.

METHODS

Different concentrations of V(C), AC, AHC, Na2S2O3 and PVP were added to the light brown suspension cells, and the contents of phenols, total coumarins, bergaptol and bergapten were determined by UV-Vis and HPLC.

RESULTS

PVP with low concentration and V(C) improved the growth of the suspension cells in different degrees. It was showed that the content of phenols in the suspension cells was related to the kinds of browning inhibitors. The addition of V(C) in the medium increased the content of total coumarins significantly. After using 2 mg/mL of V(C), the gross increase rate of total coumarins was 51.53%, which was 4.8 times than that of the control group. The browning phenomenon caused by salicylic acid were inhibited by adding 2 mg/mL of V(C) into suspension culture system (with salicylic acid as the inducer). At the same time, the content of bergaptol and bergapten was increased 25.96% and 33.33%, respectively.

CONCLUSION

V(C) is the best anti-browning agent in this study. It can inhibit browning, and promote cell growth and accumulation of secondary metabolites in Changium smyrnioides suspension cells.

摘要

目的

研究褐变抑制剂对明党参悬浮细胞生长及次生代谢产物合成的影响。

方法

向浅褐色悬浮细胞中添加不同浓度的V(C)、AC、AHC、Na2S2O3和PVP，采用紫外可见分光光度法和高效液相色谱法测定细胞中酚类、总香豆素、补骨脂酚和补骨脂素的含量。

结果

低浓度的PVP和V(C)不同程度地促进了悬浮细胞的生长。结果表明，悬浮细胞中酚类物质的含量与褐变抑制剂的种类有关。培养基中添加V(C)显著提高了总香豆素的含量。添加2mg/mL V(C)后，总香豆素的总增加率为51.53%，是对照组的4.8倍。在悬浮培养体系中添加2mg/mL V(C)（以水杨酸为诱导剂）可抑制水杨酸引起的褐变现象。同时，补骨脂酚和补骨脂素的含量分别增加了25.96%和33.33%。