Yu Ying-fang, Wu Xiu-ping, Chu Yan-hong, Chen Jia-xu, Tian Li-guang
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2016 Feb;28(1):51-3, 65.
To observe the growth situation of Blastocystis hominis in vitro and select the optimal method for cultivation of B. hominis in different media.
Ten positive stools with B. hominis were inoculated in three different media for cultivating, namely 1640, Jone's medium and vitro medium. And the stools with good growth status and high quantities of B. hominis were chosen to inoculate in the three media with equal amount after subcultivation, and the number of B. hominis was counted every 24 h for ten days, and the morphological changes and growth status were also observed.
The densities of B. hominis in the 1640 and Jone's medium were higher than that in the vitro medium 48 h after the inoculation. The same stool sample was inoculated to the three different media and observed for ten days, and the results indicated that the growth of B. hominis presented regular changes in the three media, the growth peaks were on the third, sixth and ninth day post inoculation; and the density of B. hominis was the highest in the Jone's medium. The morphology of B. hominis was the clearest and most dynamic in the vitro medium, while various reproductive forms were observed in the Jone's medium.
Jone's medium is suitable for the growth of B. hominis and can be the first choice for the cultivation of B. hominis in vitro, and vitro medium is the best medium for observing the growth situation of B. hominis.
观察人芽囊原虫在体外的生长情况，筛选其在不同培养基中培养的最佳方法。
将10份人芽囊原虫阳性粪便接种于3种不同培养基（1640培养基、琼氏培养基和体外培养基）中进行培养。选取生长状态良好、虫体数量多的粪便进行传代培养后等量接种于3种培养基中，连续10天每天24小时计数人芽囊原虫数量，并观察其形态变化和生长状态。
接种后48小时，1640培养基和琼氏培养基中人芽囊原虫密度高于体外培养基。将同一粪便样本接种于3种不同培养基中观察10天，结果显示人芽囊原虫在3种培养基中的生长呈现规律性变化，接种后第3、6和9天出现生长高峰；琼氏培养基中人芽囊原虫密度最高。体外培养基中人芽囊原虫形态最清晰、最具活力，而琼氏培养基中观察到各种生殖形态。
琼氏培养基适合人芽囊原虫生长，可作为人芽囊原虫体外培养的首选培养基，体外培养基是观察人芽囊原虫生长情况的最佳培养基。
