Tan S W, Singh M, Thong K T, Ho L C, Moe K T, Chen X Q, Ng G C, Yap E H
Department of Microbiology, Faculty of Medicine, National University of Singapore.
Parasitol Res. 1996;82(8):737-9. doi: 10.1007/s004360050194.
The present report describes a method for establishment of colonies of Blastocystis hominis from single cells in soft agar. The percentage of colony-forming efficiency (% CFE = number of colonies grown/number of cells inoculated x 100) for the cultures was greatly improved by the addition of sodium thioglycollate. Five human Blastocystis isolates chosen for this study showed no apparent variation in colonial morphology. Isolated colonies were also successfully grown in liquid medium, providing a means of obtaining large numbers of B. hominis cells that had arisen from a single clone.
本报告描述了一种在软琼脂中从单细胞建立人芽囊原虫菌落的方法。通过添加巯基乙酸钠，培养物的菌落形成效率百分比（%CFE = 生长的菌落数/接种的细胞数×100）得到了显著提高。本研究选择的5株人芽囊原虫分离株在菌落形态上没有明显差异。分离出的菌落也能在液体培养基中成功生长，提供了一种获得来自单个克隆的大量人芽囊原虫细胞的方法。
