用于检测立克次氏体属的新型环介导等温扩增（LAMP）检测方法的开发
Development of a Novel Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Rickettsia spp.
作者信息
Hanaoka Nozomu, Matsutani Minenosuke, Satoh Masaaki, Ogawa Motohiko, Shirai Mutsunori, Ando Shuji
机构信息
Department of Virology-1, National Institute of Infectious Diseases.
出版信息
Jpn J Infect Dis. 2017 Jan 24;70(1):119-123. doi: 10.7883/yoken.JJID.2015.579. Epub 2016 Jun 30.
We developed a novel loop-mediated isothermal amplification (LAMP) method to detect Rickettsia spp., including Rickettsia prowazekii and R. typhi. Species-specific LAMP primers were developed for orthologous genes conserved among Rickettsia spp. The selected modified primers could detect all the Rickettsia spp. tested. The LAMP method was successfully used to detect 100 DNA copies of Rickettsia spp. within approximately 60 min at 63℃. Therefore, this method may be an excellent tool for the early diagnosis of rickettsiosis in a laboratory or in the field.
我们开发了一种新型的环介导等温扩增（LAMP）方法来检测立克次体属物种，包括普氏立克次体和斑疹伤寒立克次体。针对立克次体属物种中保守的直系同源基因开发了物种特异性LAMP引物。所选择的修饰引物能够检测所有测试的立克次体属物种。该LAMP方法在63℃下约60分钟内成功检测到100个立克次体属物种的DNA拷贝。因此，该方法可能是实验室或现场早期诊断立克次体病的一种出色工具。
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