Withers S G, Jullien M, Sinnott M L, Viratelle O M, Yon J M
Eur J Biochem. 1978 Jun 15;87(2):249-56. doi: 10.1111/j.1432-1033.1978.tb12373.x.
The effect of pH upon the beta-galactosidase-catalyzed hydrolyses of aryl galactosides is essentially similar for each of the three steps of their hydrolysis. It differs markedly from that on the hydrolysis of galactosyl pyridinium salts; these proceed through a 'non-bottleneck' pathway. While pH increase abolishes the rate of every step of the reaction for aryl galactosides, it favors the first step of hydrolysis of the galactosyl pyridinium salts, which supports the hypothesis that catalysis of these compounds originates largely in non-covalent interactions.
pH对芳基半乳糖苷的β-半乳糖苷酶催化水解的影响，在其水解的三个步骤中本质上是相似的。这与pH对半乳糖基吡啶鎓盐水解的影响明显不同；后者通过“非瓶颈”途径进行。虽然pH升高会消除芳基半乳糖苷反应各步骤的速率，但它有利于半乳糖基吡啶鎓盐水解的第一步，这支持了这些化合物的催化作用主要源于非共价相互作用的假设。
