Ribas A, Poonlaphdecha S
Biodiversity Research Group, Faculty of Science, Udon Thani Rajabhat University, Udon Thani, Thailand.
Zoonoses Public Health. 2017 Mar;64(2):106-110. doi: 10.1111/zph.12286. Epub 2016 Jun 30.
The role of amphibians as Salmonella reservoirs has not been as well studied as in reptiles, where the literature is abundant. Recent outbreaks of salmonellosis associated with exotic pet frogs have occurred in United States. Frog farming and wild frog harvesting have increased the international trade in these species. This necessitates a better understanding of the risk of salmonellosis transmission from amphibians to humans. We explored the presence of Salmonella in amphibians (frogs and toads) in Thailand, where farmed and wild frogs as well as toads are present. These live animals are easily found in the local markets and are used as food. Exportation of frog meat from Thailand is common. During March-June 2014, ninety-seven frogs were collected from several habitats, including frog farms, urban areas and protected natural areas. The collected amphibians were tested for the presence of Salmonella. The overall prevalence of Salmonella was 69.07% (90.00% in farm animals, 0% in urban area animals and 44.83% in protected area animals). Eight serovars of Salmonella were isolated: subsp. diarizonae ser. 50:k:z, Hvittingfoss, Muenchen, Newport, Stanley, Thompson, Panama and Wandsworth. Six of the identified serovars, Hvittingfoss, Newport, Panama, Stanley, Thompson and Wandsworth, have been detected in humans in Thailand. According to our results, amphibians are reservoirs of Salmonella and can be a public health concern when used as a source of protein for humans.
两栖动物作为沙门氏菌宿主的作用尚未像爬行动物那样得到充分研究，关于爬行动物的相关文献很多。美国近期发生了与外来宠物蛙相关的沙门氏菌病疫情。青蛙养殖和野生青蛙捕捞增加了这些物种的国际贸易。这就需要更好地了解沙门氏菌病从两栖动物传播给人类的风险。我们在泰国对两栖动物（青蛙和蟾蜍）中沙门氏菌的存在情况进行了探索，泰国既有养殖青蛙和野生青蛙，也有蟾蜍。这些活体动物在当地市场很容易买到，并且被用作食物。泰国青蛙肉出口很常见。2014年3月至6月期间，从包括青蛙养殖场、城市地区和自然保护区在内的多个栖息地收集了97只青蛙。对收集到的两栖动物进行了沙门氏菌检测。沙门氏菌的总体患病率为69.07%（养殖场动物中为90.00%，城市地区动物中为0%，保护区动物中为44.83%）。分离出了8种沙门氏菌血清型：亚利桑那亚种血清型50:k:z、维廷福斯、慕尼黑、纽波特、斯坦利、汤普森、巴拿马和旺兹沃思。在泰国已在人类中检测到6种已鉴定的血清型，即维廷福斯、纽波特、巴拿马、斯坦利、汤普森和旺兹沃思。根据我们的结果，两栖动物是沙门氏菌的宿主，当被用作人类的蛋白质来源时，可能会成为公共卫生问题。
