Skog S, Ericsson A, Nordell B, Nishida T, Tribukait B
Department of Medical Radiobiology, Karolinska Institute, Stockholm, Sweden.
Acta Oncol. 1989;28(2):277-81. doi: 10.3109/02841868909111263.
The cellular ATP content and the phosphorylation potential, defined as the ATP, ADP and inorganic phosphate (Pi) ratios, of exponentially growing Ehrlich ascites tumour cells were compared with cells at the plateau phase of growth. These phosphorus compounds were measured using 31P-NMR-spectroscopy immediately after removal of the cell material from the host and in their ascites fluid reflecting in vivo growth conditions. Reaching the plateau phase of growth, the ATP content and the phosphorylation potential decreased. Upon addition of glucose, the phosphorylation potential immediately increased. We concluded that the reduced phosphorylation potential was due to a limited availability of glucose in spite of the nearly normal blood glucose concentration found. An increasing diffusion distance from the host to all parts of the tumor is a possible reason for that.
将指数生长期的艾氏腹水瘤细胞的细胞ATP含量以及定义为ATP、ADP和无机磷酸盐（Pi）比率的磷酸化电位，与处于生长平台期的细胞进行了比较。在从宿主中取出细胞材料后，立即使用31P-NMR光谱法测量这些磷化合物，并在反映体内生长条件的腹水中进行测量。进入生长平台期后，ATP含量和磷酸化电位下降。添加葡萄糖后，磷酸化电位立即升高。我们得出结论，尽管发现血糖浓度几乎正常，但磷酸化电位降低是由于葡萄糖供应有限。从宿主到肿瘤各个部位的扩散距离增加可能是其原因。
