Dell'Antone P
Department of Experimental Biomedical Sciences, University of Padova, Italy.
FEBS Lett. 1994 Aug 22;350(2-3):183-6. doi: 10.1016/0014-5793(94)00759-4.
Ehrlich ascites tumor cells were found to be in a low bioenergetic status, as evaluated by acridine orange uptake and ATP content, when resuspended in a glucose medium shortly after removal from the animal. Dye uptake as well as ATP content then increased for about 2 h at room temperature. This effect was only slightly inhibited by oligomycin. Cells resuspended in a glucose-free medium initially showed high dye uptake and ATP level, which were stable over time: in this case oligomycin caused a drop in both dye uptake and ATP level. The above findings, which are indicative of a marked Crabtree effect in Ehrlich ascites tumor cells, means that it is unlikely that limiting ADP and Pi play an important role in the glucose-induced inhibition of oxidative phosphorylation in this system.
通过吖啶橙摄取和ATP含量评估发现，艾氏腹水瘤细胞在从动物体内取出后不久重悬于葡萄糖培养基中时，处于低生物能量状态。在室温下，染料摄取以及ATP含量随后会增加约2小时。这种效应仅被寡霉素轻微抑制。重悬于无葡萄糖培养基中的细胞最初显示出高染料摄取和ATP水平，且随时间保持稳定：在这种情况下，寡霉素导致染料摄取和ATP水平均下降。上述发现表明艾氏腹水瘤细胞中存在明显的巴斯德效应，这意味着在该系统中，限制ADP和磷酸可能在葡萄糖诱导的氧化磷酸化抑制中不起重要作用。
