Bahrehmand Namaghi I, Vaziri H
Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
Andrologia. 2017 Apr;49(3). doi: 10.1111/and.12627. Epub 2016 Jun 30.
Asthenozoospermia is an important cause of male infertility. The mutations in sperm mitochondrial DNA (mtDNA) result in either functionless or malfunctioning some proteins, subsequently affecting sperm motility leading to asthenozoospermia. The purpose of this study was to investigate sperm mtDNA 4,977-bp deletion in infertile men with low sperm motility/immotile spermatozoa compared to healthy subjects with high sperm motility. Semen samples of 256 asthenozoospermic infertiles and 200 controls from northern Iran were collected. After extraction of spermatozoa total DNA, Gap-polymerase chain reaction (Gap-PCR) was performed. The deletion was observed in 85.93% of patients with asthenozoospermia compared with 14% in controls [OR = 37.5397, 95% confidence interval = 12.937-108.9276, p < .0001]. It is concluded that there is a strong association between sperm mtDNA 4,977-bp deletion and asthenozoospermia-induced infertility in the population examined. Large-scale mtDNA deletions in spermatozoa may induce bioenergetic disorders. Nevertheless, to validate our results broader research may be needed.
弱精子症是男性不育的一个重要原因。精子线粒体DNA（mtDNA）的突变会导致一些蛋白质无功能或功能异常，进而影响精子活力，导致弱精子症。本研究的目的是调查精子活力低/精子不动的不育男性与精子活力高的健康受试者相比，精子mtDNA 4977bp缺失的情况。收集了伊朗北部256例弱精子症不育患者和200例对照的精液样本。提取精子总DNA后，进行缺口聚合酶链反应（Gap-PCR）。弱精子症患者中85.93%观察到缺失，而对照组为14%[比值比=37.5397，95%置信区间=12.937 - 108.9276，p <.0001]。结论是，在所研究的人群中，精子mtDNA 4977bp缺失与弱精子症导致的不育之间存在很强的关联。精子中的大规模mtDNA缺失可能会引发生物能量紊乱。然而，为了验证我们的结果，可能需要更广泛的研究。
